Phagocytosis and Phagosomes, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1519
This volume details experimental approaches used to investigate phagocytosis and phagosome maturation. Chapters present methods and protocols on quantifying uptake and phagosome maturation using biophysical and biochemical approaches, proteomics, microscopy, and flow cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Phagocytosis and Phagosomes: Methods and Protocols aims to be an important resource for both experts in the field and for those investigators delving into phagocytosis and phagosome maturation for the first time.
Phagocytosis: Hungry, Hungry Cells.- Analysis of Human and Mouse Neutrophil Phagocytosis by Flow Cytometry.- Quantitative Efferocytosis Assays.- Quantifying Phagocytosis by Immunofluorescence and Microscopy.- Single Cell Analysis of Phagocytosis, Phagosome Maturation, Phagolysosomal Leakage and Cell Death Following Exposure of Macrophages to Silica Particles.- Quantitative Live-cell Fluorescence Microscopy during Phagocytosis.- Intracellular Manipulation of Phagosomal Transport and Maturation Using Magnetic Tweezers.- Quantitative Immunofluorescence to Study Phagosome Maturation.- Using Fluorescence Resonance Energy Transfer-Based Biosensors to Probe Rho Gtpase Activation during Phagocytosis.- Analysis of LC3-associated Phagocytosis and Antigen Presentation.- Quantitative Spatio-Temporal Analysis of Phagosome Maturation in Live Cells.- Measuring Phagosomal pH by Fluorescence Microscopy.- Image-Based Analysis of Phagocytosis – Measuring Engulfment and Internalization.- Fluorometric Approaches to Measuring Reductive and Oxidative Events in Phagosomes.- Simultaneous Analysis of Multiple Lumenal Parameters of Individual Phagosomes Using High-Content Imaging.-
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 11-2016
Ouvrage de 360 p.
17.8x25.4 cm
Ouvrage de 360 p.
17.8x25.4 cm
Thème de Phagocytosis and Phagosomes :
Mots-clés :
phagocytes; pathogens; phagosome maturation; cell fractionation; Western blotting; proteomics