Periodontal Pathogens, 1st ed. 2021 Methods and Protocols Methods in Molecular Biology Series, Vol. 2210

Part I: Methods for Bacterial Genetic Manipulation

1. Site-Directed and Random Mutagenesis in Porphyromonas gingivalis: Construction of Fimbriae-Related-Gene Mutant

            So-ichiro Nishiyama, Yoshiaki Hasegawa, and Keiji Nagano

2. Genetic Manipulations of Oral Spirochete Treponema denticola

            Kurni Kurniyati and Chunhao Li

3. Construction of a Gene-Deletion Mutant in Tannerella forsythia

            Keiji Nagano and Yoshiaki Hasegawa

4. Construction of a Mutant in Prevotella melaninogenica Using the Conjugation Transfer Method with Escherichia coli

            Yoshio Kondo

5. Genetic Transformation of Fusobacterium nucleatum

            Akihiro Yoshida and Akihiko Ikegami

Part II: Experimental Methods to Examine Virulence Factors

6. Genotyping of Porphyromonas gingivalis in Relationship to Virulence

            Atsuo Amano, Youn-Hee Choi, and Hiroki Takeuchi

7. Transport and Polymerization of Porphyromonas gingivalis Type V Pili

            Mikio Shoji, Satoshi Shibata, Mariko Naito, and Koji Nakayama

8. Purification of Native Mfa1 Fimbriae from Porphyromonas gingivalis

            Yoshiaki Hasegawa, Keiji Nagano, Yukitaka Murakami, and Richard J. Lamont

9. Crystallization of Recombinant Fimbrial Proteins of Porphyromonas gingivalis

            Thomas Heidler and Karina Persson

10. Enzymatic Characteristics and Activities of Gingipains from Porphyromonas gingivalis

            Tomoko Kadowaki

11. Structural Characterization of the Type IX Secretion System in Porphyromonas gingivalis

12. Methods for Functional Characterization of the Type IX Secretion System of Porphyromonas gingivalis

            Keiko Sato

13. Purification of Tannerella forsythia Surface (S-) Layer Proteins

            Sreedevi Chinthamani, Prasad R. Settem, Kiyonobu Honma, Takuma Nakajima, and Ashu Sharma

14. Separation of Glycosylated OmpA-Like Proteins from Porphyromonas gingivalis and Tannerella forsythia

15. Intranasal Vaccine Study Using Porphyromonas gingivalis Membrane Vesicles: Isolation Method and Application to a Mouse Model

            Satoru Hirayama and Ryoma Nakao

16. Analysis of the Butyrate-Producing Pathway in Porphyromonas gingivalis

            Yasuo Yoshida

17. Characterization of the Treponema denticola Virulence Factor Dentilisin

18. Evaluation of the Virulence of Aggregatibacter actinomycetemcomitans through the Analysis of Leukotoxin

            Toshiyuki Nagasawa, Satsuki Kato, and Yasushi Furuichi

19. Lipoprotein Extraction from Microbial Membrane and Lipoprotein/Lipopeptide Transfection into Mammalian Cells

            Akira Hasebe, Ayumi Saeki, and Ken-ichiro Shibata

Part III: Interactions with Other Pathogenic Microorganism and Host Cells

20. Analysis of the Interaction between HIV and Periodontopathic Bacteria that Reactivates HIV Replication in Latently Infected Cells

            Kenichi Imai

21. Invasion of Gingival Epithelial Cells by Porphyromonas gingivalis

            Hiroki Takeuchi and Atsuo Amano

22. Analysis of Interaction between Porphyromonas gingivalis and Endothelial Cells In Vitro

            Kenji Matsushita

Part IV: Animal Model of Periodontitis

23. Analysis of Experimental Ligature-Induced Periodontitis Model in Mice

            Hikaru Tamura, Tomoki Maekawa, Takumi Hiyoshi, and Yutaka Terao

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts