Peptidomics, Softcover reprint of the original 1st ed. 2010 Methods and Protocols Methods in Molecular Biology Series, Vol. 615

Peptidomics: Divide et Impera.- From Bacteria to Men.- Performing Comparative Peptidomics Analyses of Salmonella from Different Growth Conditions.- Approaches to Identify Endogenous Peptides in the Soil Nematode Caenorhabditis elegans.- Mass Spectrometric Analysis of Molluscan Neuropeptides.- Monitoring Neuropeptides In Vivo via Microdialysis and Mass Spectrometry.- Protocols for Peptidomic Analysis of Spider Venoms.- Purification and Characterization of Biologically ctive Peptides from Spider Venoms.- MALDI-TOF Mass Spectrometry Approaches to the Characterisation of Insect Neuropeptides.- Direct MALDI-TOF Mass Spectrometric Peptide Profiling of Neuroendocrine Tissue of Drosophila.- Direct Peptide Profiling of Brain Tissue by MALDI-TOF Mass Spectrometry.- Peptidomic Analysis of Single Identified Neurons.- Identification and Analysis of Bioactive Peptides in Amphibian Skin Secretions.- An Efficient Protocol for DNA Amplification of Multiple Amphibian Skin Antimicrobial Peptide cDNAs.- Combined Peptidomics and Genomics Approach to the Isolation of Amphibian Antimicrobial Peptides.- Identification and Relative Quantification of Neuropeptides from the Endocrine Tissues.- Peptidome Analysis of Mouse Liver Tissue by Size Exclusion Chromatography Prefractionation.- Rat Brain Neuropeptidomics: Tissue Collection, Protease Inhibition, Neuropeptide Extraction, and Mass Spectrometric Analysis.- Quantitative Neuroproteomics of the Synapse.- Peptidomics Analysis of Lymphoblastoid Cell Lines.- Peptidomics: Identification of Pathogenic and Marker Peptides.- Tools and Approaches.- Peptidomic Approaches to the Identification and Characterization of Functional Peptides in Hydra.- Immunochemical Methods for the Peptidomic Analysis of Tachykinin Peptides and Their Precursors.- Affinity Peptidomics: Peptide Selection and Affinity Capture on Hydrogels and Microarrays.- In Situ Biosynthesis of Peptide Arrays.- Bioinformatic Approaches to the Identification of Novel Neuropeptide Precursors.- Bioinformatic Identification of Plant Peptides.

Collective effort by nearly 70 scientists from over 20 leading laboratories worldwide to produce all-inclusive yet concise practical manual to peptidomics Complete: Includes all peptidomic methods in a single volume Comprehensive: Covers a wide range of species from bacterial to man Convenient: Provides step-by-step laboratory protocols with troubleshooting advice Captivating: Supplies the best practical introduction to the fascinating world of peptides Creative: Impresses with the thoroughness of the methods and the ingenuity of the experimental design Includes supplementary material: sn.pub/extras