PCR, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1620

This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field.  Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology,  and healthcare. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.

Ruslan Kalendar, Alexandr Muterko, Malika Shamekova, and Kabyl Zhambakin

2. Introduction on using the FastPCR Software and the Related Java Web Tools for PCR and Oligonucleotide Assembly and Analysis

Ruslan Kalendar and Timofey V. Tselykh

3. Long Fragment Polymerase Chain Reaction

Eng Wee Chua, Simran Maggo, and Martin A. Kennedy

4. Strategies to Improve Efficiency and Specificity of Degenerate Primers in PCR

Maria Jorge Campos and Alberto Quesada

5.  Inverse PCR for Point Mutation Introduction

Diogo Silva, Gustavo Santos, Mário Barroca, and Tony Collins

6. Synthesis of Fusion Genes for Cloning by Megaprimer-based PCR

Tatiana Q. Aguiar, Carla Oliveira, and Lucília Domingues

7. A Novel Platform for High-throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli

Ana Filipa Sequeira, Joana L.A. Brás, Vânia O. Fernandes, Catarina I.P.D. Guerreiro, Renaud Vincentelli, and Carlos M.G.A. Fontes

<8. Colony PCR

Flávio Azevedo, Humberto Pereira, and Björn Johansson

9. CRENAME, a Molecular Microbiology Method Enabling Multiparametric Assessment of Potable/Drinking Water

Luc Bissonnette, Andrée F. Maheux, and Michel G. Bergeron

10.  Multiplex Detection Of Food-borne Pathogen

Germán Villamizar-Rodríguezand Felipe Lombó

Francisco J. Santaclara and Montserrat Espiñeira

12. Fast Real-time PCR Method for the Detection of Soy in Foods

Montserrat Espiñeira and Francisco J. Santaclara

13. RAPD/SCAR Approaches for Identification of Adulterant’s Breed’s Milk in Dairy Products

14. Genetic Diversity Analysis of Medicinally Important Horticultural Crop Aegle marmelos by ISSR Markers

Farina Mujeeb, Preeti Bajpai, Neelam Pathak, and Smita Rastogu Verma

15. PCR in the Analysis of Clinical Samples: Prenatal and Postnatal Diagnosis of Inborn Errors of Metabolism

Laura Vilarinho and Célia Nogueira

16. Harnessing the Power of PCR-dependent Molecular Fingerprinting Methods for Understanding Structure and Function in Microbial Communities

S.S. Phadke, A.F.Salvador, J.I.  Alves, O. Bretschger, M.M. Alves, and M.A. Pereira

17. PCR in Metagenomics

Sarita G Bhat and Tina K. J.

Leigh Burgoyne, Lin Y Koh^, and David Catcheside

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras