Osteoporosis and Osteoarthritis (2^nd Ed., 2nd ed. 2015) Methods and Protocols Methods in Molecular Biology Series, Vol. 1226

Isolation Of Osteocytes From Mature and Aged Murine Bone.- Primary Murine Growth Plate and Articular Chondrocyte Isolation and Culture.- Isolating Endosteal Mesenchymal Progenitors From Rodent Long Bones.- Engineering Cartilage Tissue By Pellet Co-Culture Of Chondrocytes And Mesenchymal Stem Cells. - Generation Of Induced Pluripotent Stem Cells.- Profiling Histone Modifications By Chromatin Immunoprecipitation Coupled To Deep Sequencing In Skeletal Cells.- Identification Of Micrornas In Human Plasma.- Immunohistochemistry Of Skeletal Tissues.- In Vivo Axial Loading Of The Mouse Tibia.- Four Point Bending Protocols To Study The Effects Of Dynamic Strain In Osteoblastic Cells In Vitro.- Epic-µct Imaging Of Articular Cartilage.- Mouse Models Of Osteoarthritis: Surgical Model Of Post-Traumatic Osteoarthritis Induced By Destabilization Of The Medial Meniscus.- Assessment Of Knee Joint Pain In Experimental Rodent Models Of Osteoarthritis.- Induction Of Fully Stabilized Cortical Bone Defects To Study Intramembranous Bone Regeneration.- Surgical Procedures And Experimental Outcomes Of Closed Fractures In Rodent Models                                                                                                                          

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras