Odorant Binding and Chemosensory Proteins Methods in Enzymology Series
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.
- Genome mining and sequence analysis of chemosensory soluble proteins in arthropods
- Reconstructing protein coding sequences from ancient DNA
- Bioinformatic, genomic and evolutionary analysis of genes: A case study in Dipteran CSPs
- Proteomics of arthropod soluble olfactory proteins
- Analysis of post-translational modifications in soluble proteins involved in chemical communication from mammals and insects
- Bacterial expression and purification of vertebrate odorant-binding proteins
- Structure of odorant binding proteins and chemosensory proteins determined by X-ray crystallography
- Solution structure of insect CSP and OBPs by NMR
- Stability of OBPs
- Ligand-binding assays with OBPs and CSPs
- Fine structure immunocytochemistry—An important tool for research on odorant-binding proteins
- Effect of OBPs on the response of olfactory receptors
- Site-directed mutagenesis of odorant-binding proteins
- Silencing of OBP genes: Generation of loss-of-function mutants of PBP by genome editing
- Electrochemical biosensing with odorant binding proteins
- Electronic biosensors based on graphene FETs
- Electronic biosensors based on EGOFETs
- Gravimetric biosensors
- Plasmonic biosensors relying on biomolecular conformational changes: Case of odorant binding proteins
Joel Vizueta, Paula Escuer, Alejandro Sánchez-Gracia and Julio Rozas
Michael Hofreiter and Stefanie Hartmann
Jean-François Picimbon
Francesca R. Dani and Giuseppe Pieraccini
Andrea Scaloni
Marine Brulé, Margot Glaz, Christine Belloir, Nicolas Poirier, Lucie Moitrier, Fabrice Neiers and Loïc Briand
Anaïs Gaubert, Béatrice Amigues, Silvia Spinelli and Christian Cambillau
Serena Leone, Alessandro Emendato, Roberta Spadaccini and Delia Picone
Nadja Hellmann
Chiara D'Onofrio, Valeriia Zaremska, Jiao Zhu, Wolfgang Knoll and Paolo Pelosi
Rudolf Alexander Steinbrecht
Bing Wang, Song Cao, Wei Liu and Guirong Wang
Jiao Zhu, Valeriia Zaremska, Chiara D'Onofrio, Wolfgang Knoll and Paolo Pelosi
Yusuke Shiota and Takeshi Sakurai
Sabine Szunerits, Rabah Boukherroub and Alina Vasilescu
Patrik Aspermair, Vladyslav Mishyn, Sabine Szunerits and Wolfgang Knoll
Mohammad Y. Mulla, Luisa Torsi and Kyriaki Manoli
Khasim Cali, Elena Tuccori and Krishna C. Persaud
Anil Bozdogan, Simone Hageneder and Jakub Dostalek
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology series
Date de parution : 08-2020
Ouvrage de 514 p.
15x22.8 cm
Mots-clés :
1-Aminoanthracene; Affinity; Apollo browser; BED files; Biosensing; Biosensor; BITACORA pipeline; BLASTP; Chemoreception; Chemosensory proteins; Chemosensory sensilla; Circular dichroism; Conformation change; CPSs; Crystallization; CSP-sg4 structure; Disulfide; Electrochemical sensing; Electron microscopy; Electronic nose; Electronic smell sensing; Fluorescence; Fluorescent assay; Gene family; Genome sequence; gFET: graphene field-effect transistors; GFF files; Glycosylation; HMM profiles; Immunolabeling; Insect antennae; Insect CSP; Insect OBP; Interplay; Isothermal microcalorimetry; Kinetic analysis; Label-free; Langmuir adsorption model; Ligand-binding; Lipocalin; Mass spectrometry; Metallic nanostructure; Micelles; NPC2 proteins; N-phenyl-1-naphthylamine; OBP: odorant-binding protein; OBPs; Odorant; Odorant binding protein; Odorant binding proteins; Odorant binding proteins (OBP); Odorant-binding protein; Odorant-binding proteins; Olfactory receptors; Pheromone binding proteins; Pheromone-binding protein; Plasmon-enhanced fluorescence; Plasmonics; Post-translational modifications; Protein NMR; Purification; Sex pheromones; Shotgun proteomics; Small molecule analysis; Surface functionalization; Surface plasmon resonance; Titration analysis; Two-electrode voltage-clamp recording; Xenopus laevis oocytes; X-ray diffraction
