Myeloid-Derived Suppressor Cells, 1st ed. 2021 Methods in Molecular Biology Series, Vol. 2236

Preface…
Table of Contents...
Contributing Authors...

1. Immunophenotyping of Circulating Myeloid-Derived Suppressor Cells (MDSC) in the Peripheral Blood of Cancer Patients
Kirsten Bruderek, Ronja Schirrmann, and Sven Brandau

2. Phenotyping of Myeloid-Derived Suppressor Cells in Nonhuman Primates
Ang Lin and Karin Loré 

3. Isolation and Phenotyping of Splenic Myeloid-Derived Suppressor Cells in Murine Cancer Models
Emilio Sanseviero, Rina Kim, and Dmitry I. Gabrilovich

4. Phenotypical Characterization and Isolation of Tumor-Derived Mouse Myeloid-Derived Suppressor Cells
Roza Maria Barouni, Chiara Musiu, Vincenzo Bronte, Stefano Ugel, and Stefania Canè

5. Isolation of Human Circulating Myeloid-Derived Suppressor Cells and Analysis of Their Immunosuppressive Activity
Kirsten Bruderek, Ronja Schirrmann, and Sven Brandau

6. High Dimensional Phenotyping of Human Myeloid-Derived Suppressor Cells/Tumor-Associated Macrophages in Tissue by Mass Cytometry
Juliette Ferrant, Simon Le Gallou, Guillaume Manson, Steve Genebrier, Frederic Mourcin, Karin Tarte, and Mikael Roussel

7. Depletion and Maturation of Myeloid-Derived Suppressor Cells in Murine Cancer Models
Christopher Groth, Rebekka Weber, Jochen Utikal, and Viktor Umansky

8. In Vitro Generation of Human Neutrophilic Myeloid-Derived Suppressor Cells 
Anurag Singh and Nikolaus Rieber

9. Measuring Suppressive Activity and Autophagy in Myeloid-Derived Suppressor Cells
Antonis Stylianos Papaioannou, Athina Boumpas, Miranta Papadopoulou, Aikaterini Hatzioannou, Themis Alissafi, and Panayotis Verginis 

10. In Vitro Generation of Murine Myeloid-Derived Suppressor Cells, Analysis of Markers, Developmental Commitment, and Function
Ina Eckert, Eliana Ribechini, and Manfred B. Lutz

11. Analysis of Antimicrobial Activity of Monocytic Myeloid-Derived Suppressor Cells in Infection with Mycobacterium tuberculosis and Human Immunodeficiency Virus
Ankita Garg

12. Isolation and Functional Characterization of Myeloid-Derived Suppressor Cells in Infections Under High Containment
Leigh A. Kotze, Vinzeigh N. Leukes, and Nelita du Plessis

13. High-Dimensional Analysis of Circulating and Tissue-Derived Myeloid-Derived Suppressor Cells from Patients with Glioblastoma
Tyler J. Alban, Defne Bayik, Alvaro G. Alvarado, Harley I. Kornblum, and Justin D. Lathia

14. Single Cell Transcriptome Analysis Workflow for Splenic Myeloid-Derived Suppressor Cells from Murine Breast Cancer Models
Hamad Alshetaiwi, Nicholas Pervolarakis, Quy H. Nguyen, and Kai Kessenbrock

15. Intravital 2-Photon Microscopy of Diverse Cell Types in the Murine Tibia
Anja Hasenberg, Lucas Otto, and Matthias Gunzer

16. Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting
Marion Rambault, Rachana Borkute, Emilie Doz-Deblauwe, Yves Le-Vern, Nathalie Winter, Anca Dorhoi, and Aude Remot

Subject Index List…

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts