Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols (3^rd Ed., 3rd ed. 2020) Cutting-Edge Technologies and Advancements Methods in Molecular Biology Series, Vol. 2069

This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens. 

Preface…

Table of Contents…

Contributing Authors…

1. Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections

Elizabeth L. Palavecino

Elizabeth L. Palavecino

 

3. Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus

Yekaterina Timofeyeva, Ingrid L. Scully, and Annaliesa S. Anderson

4. Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA

Tetsuo Yamaguchi, Daisuke Ono, and Ayami Sato

5. Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains

Yiping He and Sue Reed

Xin Wang

7. Multilocus Sequence Typing of Staphylococcus aureus

Ying Liu and Yinduo Ji

8. Genetic Manipulations of Staphylococcal Chromosomal DNA

Crystal M. Austin and Jeffrey L. Bose 

9. Genetic Manipulation of MRSA using CRISPR/Cas9 Technology

Weizhong Chen and Quanjiang Ji 

10. RNA-seq Identification of Target Genes Mediated by Regulators of Staphylococcus aureus

Ting Lei, Junshu Yang, Aaron Becker, and Yinduo Ji

11. Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus

Emilia Bonar, Kinga Chlebicka, Grzegorz Dubin, and Benedykt Wladyka

12. Identification of Virulence Determinants during Host-Pathogen Interaction using Tn-Seq Technology

Christopher T. Peek, Carolyn B. Ibberson, and James E. Cassat

13. Metabolomic Profiling of Staphylococcus aureus

Ting Lei, Qingqing Mao, Chi Chen, and Yinduo Ji

14. Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells

Nansea Ji, Junshu Yang, and Yinduo Ji

15. Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections using Noninvasive Optical Imaging

Nathan K. Archer, Yu Wang, Roger V. Ortines, Haiyun Liu, Sabrina J. Nolan, Qi Liu, Martin P. Alphonse, Dustin A. Dikeman, Momina Mazhar, Robert J. Miller, Leif S. Anderson, Kevin P. Francis, Scott I. Simon, and Lloyd S. Miller

16. Methicillin-Resistant Staphylococcus aureus Infection and Treatment Options

Sarah J. Chalmers, M.D. and Mark E. Wylam 

17. Animal Models for Drug Development for MRSA

Andrea Marra