Preface…
Table of Contents…
Contributing Authors…
1. Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer
Lukáš Najdekr, Giovanny Rodriguez Blanco, and Warwick B. Dunn
2. Isotopic Ratio Outlier Analysis (IROA) for Quantitative Analysis
Chris Beecher and Felice de Jong
3. Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals
Mukundan Ragavan and Matthew E. Merritt
4. Quantitative Metabolomics using Isotope Residue Outlier Analysis (IROA®) with Internal Standards
Roberto Mendez, Maria del Carmen Piqueras, Alexander Raskind, Felice de Jong, Chris Beecher, Sanjoy Bhattacharya, and Santanu Banerjee
5. Analyses of Cholesterol Metabolites of Optic Nerve using GC-MS Methods
Gurkaran S. Sarohia, Erik D. Garza, and Sanjoy K. Bhattacharya
6. Analyses of Cholesterol and Derivatives in Ocular Tissues using LC-MS/MS Methods
Maria Piqueras, Despoina Theotoka, Gurkaran Sarohia, and Sanjoy K. Bhattacharya
7. Measurement of Pyridine Nucleotides in Biological Samples using LC-MS/MS
Chris Petucci, Jeffrey A. Culver, Nidhi Kapoor, Hampton Sessions, Daniela Divlianska, and Stephen J. Gardell
8. Handling and Analysis of 5-Formyl-, 5,10-Methenyl-, 10-Formyl-, 5-Formimno-, and 5,10-Methylenetetrahydrofolates
Anita H. Lewin and Peter Silinski
9. Protein-Lipid Complex Separation Utilizing a Capillary Electrophoresis System
Anddre Osmar Valdivia, Ciara Myer, Maria Fernanda Suarez, and Sanjoy K. Bhattacharya
10. Immature and Mature Collagen Crosslinks Quantification using High Performance Liquid Chromatography and High Resolution Mass Spectrometry in OrbitrapTM
Diana R. Hernandez, Maria del Carmen Piqueras, Alejandro E. Macias, Laisel Martinez, Roberto Vazquez-Padron, and Sanjoy K. Bhattacharya
11. Relative Quantitation of Neurotoxic Metabolites of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS)
Pallavi Lahiri, Deepika Dhaware, Ajeet Singh, Venkat Panchagnula, and Dipankar Ghosh
12. Analysis of Microbial Siderophores by Mass Spectrometry
Tomas Pluhacek, Anton Skriba, Jiri Novak, Dominika Luptakova, and Vladimir Havlicek
13. HSD18B7 Enzyme Assay Technique using a Triple Quadrupole Mass Spectrometer
Carina Wu, Nhuong-Sao Ton, Maria Fernanda Suarez, Maria Piqueras Soria, and Sanjoy K. Bhattacharya
14. Assays for Intracellular Cyclic Adenosine Monophosphate (cAMP) and Lysosomal Acidification
Chiranjit Maity, Dipankar Ghosh, and Sonia Guha
15. Isotopic Ratio Outlier Analysis (IROA) of Aqueous Humor for Metabolites
Maria Piqueras, Ciara Myer, Anna Junk, and Sanjoy K. Bhattacharya
16. Assessment of Transport of Lipid Metabolites within Trabecular Meshwork Cells
Daphne Toglia, Stephanie Dancausse, Karthik Kalahasty, Bryan Alfonso, and Sanjoy K. Bhattacharya
17. Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes
Shamroop Kumar Mallela, Devang Maheshkumar Patel, Gloria Michelle Ducasa, Sandra Merscher, Alessia Fornoni, Hassan Al-Ali
18. Labeling Cancer Stem Cells with 13C6 Glucose and 13C5 Glutamine for Metabolic Flux Analysis
Kousik Kesh and Sulagna Banerjee
19. Protocols for Metabolomic Analyses from Tissues in Parkinson’s Disease
Jade Woods, Shulei Lei, Jordan Rose, Darrell D. Marshall, Fatema Bhinderwala, Eli Riekeberg, Aline De Lima Leite, Martha Morton, Eric D. Dodds, Rodrigo Franco, and Robert Powers
20. Application of Stable Isotope Labels for Metabolomics in Studies in Fatty Liver Disease
Patrycja Puchalska and Peter A. Crawford
21. Proteometabolomics of Melphalan Resistance in Multiple Myeloma
David C. Koomen, Joy D. Guingab-Cagmat, Paula S. Oliveira, Bin Fang, Min Liu, Eric A. Welsh, Mark B. Meads, Tuan Nguyen, Laurel Meke, Steven A. Eschrich, Kenneth H. Shain, Timothy J. Garrett, and John M. Koomen
22. Metabolomic Analyses of Muscle Atrophy Induced by Hindlimb Unloading
Stephen J. Gardell, Xiaolei Zhang, Nidhi Kapoor, Christopher Petucci, and Paul M. Coen
23. Protocols for NMR Analysis in Livestock Metabolomics
Aidin Foroutan, Seyed Ali Goldansaz, Matthias Lipfert, and David S. Wishart
24. Metabolomics Applicable to Retinal Vascular Diseases
Satish Patnaik, Subhadra Jalali, Manjunath B. Joshi, Kapaettu Satyamoorthy, Inderjeet Kaur