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Metabolomics Tools for Natural Product Discovery, 2013 Methods and Protocols Methods in Molecular Biology Series, Vol. 1055

Langue : Anglais

Coordonnateurs : Roessner Ute, Dias Daniel Anthony

Couverture de l’ouvrage Metabolomics Tools for Natural Product Discovery

Classical natural product chemistry is transitioning to modern day metabolomics as a result of the advent of comprehensive analytical platforms and sensitive analytical instrumentation. Therefore, it is worthwhile to summarize recent developments with current analytical platforms and highlight how metabolomics is being integrated into this classical field to dereplicate and profile natural product extracts. Metabolomics Tools for Natural Product Discoveries: Methods and Protocols aims to unite diverse and recently developed methodologies and protocols in order to identify bioactive secondary metabolites for the purpose of drug discovery. Some topics covered in this volume include applications for the extraction of selected natural products from less common sources such as bryophytes and hard corals, various biological assays, comprehensive applications and strategies for GC-MS, LC-MS, and NMR, as well as protocols and strategies for the structure elucidation of isolated natural products.  Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible Metabolomics Tools for Natural Product Discoveries: Methods and Protocols seeks to serve both professionals and research students with its well-honed methodologies for natural product isolation, biomarker discovery, dereplication, biological assays, and comprehensive metabolomic platforms available for high-throughput analyses.

1. Bryophytes: Liverworts, Mosses and Hornworts – Extraction and Isolation Procedures

                Yoshinori Asakawa and Agnieszka Ludwiczuk


2. Plant Tissue Extraction for Metabolomics

                Ute Roessner and Daniel Anthony Dias


3. Detection of Polar Metabolites through the use of Gas Chromatography – Mass Spectrometry

                David P. De Souza


4. A Robust GC-MS Method for the Quantitation of Fatty Acids in Biological Systems

                Nirupama Samanmalie Jayasinghe and Daniel Anthony Dias


5. A Workflow from Untargeted LC-MS Profiling to Targeted Natural Product Isolation

                Damien L. Callahan and Candace E. Elliot


6. Lipidomics –Extraction Protocols for Biological Matrices

                Thusitha Wasntha Thilanka Rupasinghe


7. Metabolite Analysis of Biological Fluids and Tissues by Proton Nuclear Magnetic Resonance Spectroscopy

                John Robert Sheedy


8. NMR Spectroscopy: Structure Elucidation of Cycloelatenene A: A Natural Product Case Study

                Sylvia Urban and Daniel Anthony Dias

9. NMR-Based Metabolomics: A Probe to Utilize Biodiversity

                Lúcia P. Santos Pimenta, Hye Kyong Kim, Robert Verpoorte, and Young Hae Choi             


10. Extraction Protocol for Non-Targeted NMR and LC-MS Metabolomics-Based Analysis of Hard Coral and their Algal Symbionts

                Benjamin R. Gordon, William Leggat, and Cherie Motti


11. Determination of Absolute Configuration Using Single Crystal X-ray Diffraction

                Abigail L. Albright and Jonathan M. White


12. Natural Product Chemistry in Action:  The Synthesis of Melatonin Metabolites K1 and K2

                Helmut M. Hügel and Oliver A.H. Jones


13. Discovery, Biosynthesis, and Rational Engineering of Novel Enterocin and Wailupemycin Polyketide Analogues

                John A. Kalaitziz


14. Bioassays for Anticancer Activities

                Janice McCauley, Ana Zivanovic, and D. Skropeta


15. Screening for Anti-Diabetic Activities

                Rima Caccetta and Hani Al Salami


16. Screening for Antibacterial, Antifungal and Anti-Quorum Sensing Activity

                Elisa J. Hayhoe and Enzo A. Palombo


17. Metabolomics and Dereplication Strategies in Natural Products

                Ahmed Fares Tawfike, Christina Viegelmann, and RuAngelie Edrada-Ebel


18. Bridging the Gap: Basic Metabolomics Methods for Natural Product Chemistry

                Oliver A.H. Jones and Helmut M. Hügel


19. Strategies in Biomarker Discovery. Peak Annotation by MS and Targeted LC-MS Micro-Fractionation for de novo Structure Identification by Micro-NMR

                Philippe J. Eugster, Gaëtan Glauser, and Jean-Luc Wolfender


20. Statistical Analysis of Metabolomics Data

                Alysha M. De Livera, Moshe Olshansky, and Terence P. Speed

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Date de parution :

Ouvrage de 311 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

Prix indicatif 116,04 €

Ajouter au panier

Date de parution :

Ouvrage de 311 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

Prix indicatif 179,34 €

Ajouter au panier

Thèmes de Metabolomics Tools for Natural Product Discovery :

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