Membrane Trafficking (2^nd Ed., Softcover reprint of the original 2nd ed. 2015) Second Edition Methods in Molecular Biology Series, Vol. 1270

This second edition explores membrane trafficking research that has been at the frontier of cell and molecular biology. In the past 20 years, this field has witnessed a remarkable convergence and synergism from information gained using genetic approaches in yeast cells, and biochemical and molecular approaches in mammalian cells. This volume contains updated chapters and the inclusion of a large number of new chapters, and is divided into three parts. Part I includes biochemical and molecular genetics approaches and methods used in analysing membrane traffic, in both yeast and mammalian cell models. Part II focuses on imaging and microscopy approaches and techniques, while Part III highlights the Omics-type approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and resourceful, Membrane Trafficking, Second Edition, is a valuable resource for both experienced researchers, graduate students, and junior scientists in the field of membrane trafficking.

1. Intracellular Parcel Service: Current Issues in Intracellular Membrane Trafficking
Johannes M. Herrmann and Anne Spang

Part I Biochemical and Molecular Genetics Approaches

Part IA Yeast and Protozoa Models

2. In vitro Analysis of the Mitochondrial Preprotein Import Machinery Using Recombinant Precursor Polypeptides
Dorothea Becker and Wolfgang Voos

3. Import of Proteins into Isolated Yeast Mitochondria
Valentina Peleh, Ajay Ramesh, and Johannes M. Herrmann

4. Evaluation of Unconventional Protein Secretion in Saccharomyces cerevisiae
Natsuko Miura and Mitsuyoshi Ueda

5. Fractionation of Plasmodium-infected Human Red Blood Cells to Study Protein Trafficking
Simone Külzer, Verena Bittl, and Jude M. Przyborski

Part IB Mammalian models

6. Investigating Signalling Processes in Membrane Trafficking
Laura J. Sharpe and Andrew J. Brown

7. Recruitment of Coat Proteins to Liposomes and Peptidoliposomes
Sonja Huser, Gregor Suri, Pascal Crottet, and Martin Spiess

8. A β-lactamase Based Assay to Measure Surface Expression of Membrane Proteins
Pieter Beerepoot, Vincent M. Lam, and Ali Salahpour 

9. Cell-free Reconstitution of Multivesicular Body (MVB) Cargo Sorting
Monica Gireud, Natalie Sirisaengtaksin, Susan Tsunoda and Andrew J. Bean

10. The Analysis of the Biogenesis of Lipid Droplets by Examining Rab40c Associating With Lipid Droplets
Ran Tan, Xiaohui Xu, Wanjin Hong, and Tuanlao Wang

11. Analysis of Conventional and Unconventional Trafficking of CFTR and Other Membrane Proteins
Heon Yung Gee, Joo Young Kim, and Min Goo Lee

12. Assessing Mammalian Autophagy
Sharon A. Tooze, Hannah C. Dooley, Harold B.J. Jefferies, Justin Joachim, Delphine Judith, Christopher A. Lamb, Minoo Razi, and Martina Wirth

13. Expression of Functional Myc-tagged Conserved Oligomeric Golgi (COG) Subcomplexes in Mammalian Cells
Rose A. Willett, Tetyana A. Kudlyk, and Vladimir V. Lupashin

14. Molecular and Cellular Characterization of GCC185, a Tethering Protein of the trans-Golgi Network
Pak-yan Patricia Cheung and Suzanne R. Pfeffer

15. Visualizing Toll-like Receptor-dependent Phagosomal Dynamics in Murine Dendritic Cells Using Live Cell Microscopy
Adriana Mantegazza and Michael Marks

16. Understanding of Complex Protein Interactions with Respect to Anchorage Independence
Jill M. Tham, Sofie C. Van Huffel, and Wanjin Hong

17. Application of Flow Cytometry to Analyse Intracellular Location and Trafficking of Cargo in Cell Populations
Wei Hong Toh, Fiona J. Houghton, Pei Zhi Cheryl Chia, Yasmin M. Ramdzan, Danny M. Hatters, and Paul A. Gleeson

18. Approaches to Analyze the Role of Rab GTPases in Endocytic Trafficking of Epidermal Growth Factor Receptor (EGFR)  
Christelle En Lin Chua, Angeline Yim Lim Yim Kiat, and Bor Luen Tang

PART II Microscopy Methods and Imaging Approaches

19. Does Super Resolution Fluorescence Microscopy Obsolete Previous Microscopic Approaches to Protein Co-localization?Laura MacDonald, Giulia Baldini, and Brian Storrie

20. Bimolecular Fluorescence Complementation (BiFC) Technique in Yeast Saccharomyces cerevisiae and Mammalian Cells
Marion Weber, Shiqian Li, Kari-Pekka Skarp, Vesa M. Olkkonen, Daoguang Yan, and Jussi Jäntti

21. Microscopic and Spectroscopic Techniques to Investigate Lipid Droplet Formation and Turnover in Yeast
Heimo Wolinski and Sepp D. Kohlwein

22. Image-based Identification of Nuclear Export Inhibitors From Natural Products
Bastien Cautain, Nuria de Pedro, Fernando Reyes, and Wolfgang Link

23. Correlative Video-light-electron Microscopy of Mobile Organelles
Alexander A. Mironov, and Galina V. Beznoussenko

24. Live Cell Imaging of Endosomal Trafficking in Fungi
Sebastian Baumann, Norio Takeshita, N. Grün, Reinhard Fischer, and Michael Feldbrügge

PART III Omics Approaches

26. Identification of Factors Regulating MET Receptor Endocytosis by High Throughput siRNA Screening
Ivana Gaziova, Robert A. Davey, and Lisa A. Elferink

27. Large-scale Analysis of Membrane Transport in Yeast Using Invertase Reporters
Lauren Dalton, Michael Davey, and Elizabeth Conibear

28. RNAi Screens for Genes Involved in Golgi Glycosylation
Germaine Y. Goh and Frederick A. Bard

29. Proteomics Analyses of an Unique Bi-lobed Structure in Trypanosoma brucei
Ladan Gheiratmand and Cynthia Y. He

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras