Lymph node (LN) fine-needle cytology (FNC) coupled with flow cytometry immunophenotyping provides important information for diagnosing non-Hodgkin lymphoma. This special topic issue offers comprehensive reviews that describe the state of the art of LN FNC-FC and all its possible applications. FNC and FC are two independent procedures that can conveniently meet and merge in specific conditions and for specific purposes. FNC samples are, for example, suitable for various molecular procedures. The issue includes a brief description of available tools and their potential application to LN FNC. FNC may be useful for diagnosing lymphoma and determining prognosis. As such, this publication is highly recommended to cytopathologists, pathologists, and hematologists.