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Immunocytochemistry. A practical guide for biomedical research (POD), 2010 A Practical Guide for Biomedical Research

Langue : Français

Auteur :

Couverture de l’ouvrage Immunocytochemistry. A practical guide for biomedical research (POD)
Immunocytochemistry is a microscopic method that uses antibodies to localize proteins in tissue sections and cell cultures. Dr. Burry's book is the first practical guide to planning, performing, and evaluating immunocytochemical experiments designed for novice immunocytochemists. This book's predominant orientation is academic, focusing only on the most productive methods, with a primary emphasis on animal-based research tissues. The clear descriptions of the methods are easily followed and the specific design charts show how to plan an experiment. Included are a series of experimental cases exploring actual mistakes that demonstrate the approaches presented. Immunocytochemistry is no longer considered a specialized technique - Successful Immunocytochemistry in Biomedical Research showcases this mainstream morphological approach in an accessible and exciting manner.
Chapter 1- Introduction. Chapter 2- Antibodies. Chapter 3- Sample Preparation/Fixation. Chapter 4- Tissue Embedding. Chapter 5- Blocking and Permeability. Chapter 6- Labels for Antibodies. Chapter 7- Application Methods. Chapter 8- Controls. Chapter 9- Method and Label Decision. Chapter 10- Single Antibody Procedure. Chapter 11- Multiple Antibodies Different Species. Chapter 12- Multiple Antibodies Same Species. Chapter 13- Microscopy and Images. Chapter 14- Trouble-Shooting. Chapter 15- Electron Microscopic Immunocytochemistry.

The first practical guide to planning, performing, and evaluating immunocytochemical experiments

Emphasis on practical, rather than academic, focusing only on the most productive methods

Strong focus on animal research over human pathology, as processing for animal-based tissues takes about a third of the time and results in higher quality images

Because this field is new and is emerging, there are numerous advantages of specific methods over other, more generalized methods

The purpose of this book is to show a novice how to do immunocytochemistry without engaging in a discussion of possible advanced methods

Date de parution :

Ouvrage de 223 p.

15.5x23.5 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

Prix indicatif 73,93 €

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Date de parution :

Ouvrage de 224 p.

15.5x23.5 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

Prix indicatif 89,66 €

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