Handbook of Dynein (Second Edition) (2^nd Ed.)

Dyneins are molecular motors that are involved in various cellular processes, such as cilia and flagella motility, vesicular transport, and mitosis. Since the first edition of this book was published in 2012, there has been a significant breakthrough: the crystal structures of the motor domains of cytoplasmic dynein have been solved and the previously unknown details of this huge and complex molecule have been unveiled. This new edition contains 14 chapters written by researchers in the US, Europe, and Asia, including 3 new chapters that incorporate new fields. The other chapters have also been substantially updated. Compared with the earlier edition, this book focuses more on the motile mechanisms of dynein, especially by biophysical methods such as cryo-EM, X-ray crystallography, and single-molecule nanometry. It is a major handbook for frontline researchers as well as for advanced students studying cell biology, molecular biology, biochemistry, biophysics, and structural biology.

Dyneins: Ancient Protein Complexes Gradually Reveal Their Secrets. Structural and Functional Analysis of the Dynein Motor Domain. Electron Microscopy Studies of Dynein: From Subdomains to Microtubule-Bound Assemblies. Subunit Architecture of the Cytoplasmic Dynein Tail. Measuring the Motile Properties of Single Dynein Molecules. Mechanics of Dynein: Motility. Interactions of Multiple Dynein Motors Studied Using DNA Scaffolding. Cytoplasmic Dynein Force Regulation in Vitro and in Vivo. Dynein in Endosome and Phagosome Maturation. Dynein in Intraflagellar Transport.Diversity of Chlamydomonas Axonemal Dyneins. Motility of Axonemal Dyneins Axonemal Dyneins in Cilia and Flagella. Regulatory Mechanism of Axonemal Dynein.

Keiko Hirose is a researcher with more than 30 years’ experience in structural and functional studies of motor proteins. She has a PhD from the University of Tokyo and was a postdoctoral fellow at the University of Pennsylvania and at the MRC Laboratory of Molecular Biology, Cambridge, UK. She is especially interested in how molecular motor proteins, such as dynein, move. Dr. Hirose has been working at the National Institute of Advanced Industrial Science and Technology, Japan, since 1997.