Genomic protocols (methods in molecular biology/175), 2001 Methods in Molecular Biology Series, Vol. 175

Genomics covers the broad field involving the study of genes and their functions. Recent advances in genomics have ushered in a revolution in our understanding of the molecular mechanisms of disease, including the complex interplay of genetic and environmental factors. The genomics techniques used by scientists provide innovate ways to design new drugs, vaccines and diagnostics. In this book, experienced practitioners provide their step-by-step reproducible protocols for working in the genomics field.

Construction of Microsatellite-Based, High-Resolution Genetic Maps in the Mouse Paul A. Lyons Genetic Analysis of Complex Traits Stephen P. Bryant and Mathias N. Chiano Sequence-Based Detection of Single Nucleotide Polymorphisms Deborah A. Nickerson, Natali Kolker, Scott L. Taylor, and Mark J. Rieder Genomic Mismatch Scanning for the Mapping of Genetic Traits Farideh Mirzayans and Michael A. Walter Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization Mario A. J. A. Hermsen, Marjan M. Weiss, Gerrit A. Meijer, and Jan P. A. Baak Construction of a Bacterial Artificial Chromosome Library Sangdun Choi and Ung-Jin Kim Contiguation of Bacterial Clones Sean J. Humphray, Susan J. Knaggs, and Ioannis Ragoussis Mapping of Genomic Clones by Fluorescence In Situ Hybridization Margaret A. Leversha Map Integration: From a Genetic Map to a Physical Gene Map and Ultimately to the Sequence Map Panagiotis Deloukas Construction of Full-Length-Enriched cDNA Libraries: The Oligo-Capping Method Yutaka Suzuki and Sumio Sugano Construction of Transcript Maps by Somatic Cell/Radiation Hybrid Mapping: The Human Gene Map Panagiotis Deloukas Preparation and Screening of High-Density cDNA Arrays with Genomic Clones Günther Zehetner, Maria Pack, and Katja Schäfer Direct Selection of cDNAs by Genomic Clones Daniela Toniolo Exon Trapping: Application of a Large-Insert Multiple-Exon-Trapping System Martin C. Wapenaar and Johan T. Den Dunnen Sequencing Bacterial Artificial Chromosomes David E. Harris and Lee Murphy Finding Genes in Genomic Nucleotide Sequences by Using Bioinformatics Yvonne J. K. Edwards and Simon M. Brocklehurst Gene Identification Using the Pufferfish, Fugu rubripes, by Sequence Scanning GregElgar Isolation of Differentially Expressed Genes Through Subtractive Suppression Hybridization Oliver Dorian von Stein Isolation of Differentially Expressed Genes by Representational Difference Analysis Christine Wallrapp and Thomas M. Gress Expression Profiling and the Isolation of Differentially Expressed Genes by Indexing-Based Differential Display Michael P. Starkey Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos Nicolas Pollet and Christof Niehrs Expression Monitoring Using cDNA Microarrays: A General Protocol Xing Jian Lou, Mark Schena, Frank T. Horrigan, Richard M. Lawn, and Ronald W. Davis Prediction of Protein Structure and Function by Using Bioinformatics Yvonne J. K. Edwards and Amanda Cottage Identification of Novel Genes by Gene Trap Mutagenesis Anne K. Voss and Tim Thomas Determination of Gene Function by Homologous Recombination Using Embryonic Stem Cells and Knockout Mice Ahmed Mansouri Genomic Analysis Utilizing the Yeast Two-Hybrid System Ilya G. Serebriiskii, Garabet G. Toby, Russell L. FInley, Jr., and Erica A. Golemis Methods for Adeno-Associated Virus-Mediated Gene Transfer into Muscle Terry J. Amiss and Richard Jude Samulski Retroviral-Mediated Gene Transduction Donald S. Anson Gene Therapy Approaches to Sensitization of Human Prostate Carcinoma to Cisplatin by Adenoviral Expression of p53 and by Antisense Jun Kinase Oligonucleotide Methods Ruth Gjerset, Ali Haghighi, Svetlana Lebedeva, and Dan Mercola Ribozyme Gene Therapy Leonidas A. Phylactou