G Protein-Coupled Receptors, Part B (2^nd Ed.)

G-Protein-Coupled Receptors, Part B, 2nd Edition, Volume 149, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers Optical Approaches for Visualization of Arrestin Binding to Muscarinic Receptors, Luciferase Reporter Assay for Unlocking Ligand-mediated Signaling of GPCRs, Assays to Measure GPCR Dependent Cellular Migration, Characterization of the Frizzled GPCRs, Binding Assays for Bradykinin and Angiotensin Receptors, Detection of Misfolded Rhodopsin Aggregates in Cells, Measuring GPCR Ubiquitination and Trafficking, Culture of Primary Neurons and its Use in Studying GPCR Trafficking, and much more.

1. Optical approaches for visualization of arrestin binding to muscarinic receptor Seung Ryoung Jung 2. Luciferase Reporter Assay for Unlocking Ligand-mediated Signaling of GPCRs Hamiyet Unal 3. Assays to measure GPCR dependent cellular migration Carole A. Parent 4. Characterization of the Frizzled GPCRs Vladimir L. Katanaev 5. Binding assays for bradykinin and angiotensin receptors Renan Martin 6. Detection of misfolded rhodopsin aggregates in cells Paul Park 7. Measuring GPCR ubiquitination and trafficking Samarjit Bhattacharyya 8. Culture of primary neurons and its use in studying GPCR trafficking Prem N. Yadav 9. ELISA based assay for measuring agonist-induced GPCR endocytosis Arun K. Shukla 10. Western blot assay for measuring agonist-induced ERK1/2 phosphorylation Arun K. Shukla 11. Understanding GPCR dimerization Agata Faron-Górecka 12. Investigating the Frizzled family of receptors Vladimir L. Katanaev 13. Measuring GPCR signaling through xCELLigence system based assay Laura Heitman 14. Novel mechanisms of GPCR signaling and bias Gabriel Carmona Rosas 15. co-IP assays to measure GPCR-arrestin interactions Anastasios Lymperopoulos 16. Investigating arrestin dependent GPCR signaling Sadashiva Karnik 17. Measuring CRH1 receptor signaling Susana Silberstein 18. Site-specific side chain labeling for NMR studies of G protein-coupled receptors Brian F. Volkman 19. Modeling chemokine receptors and their orthosteric site interactions Brian F. Volkman 20. Receptor-channel cross-talk Uri Kahanovitch 21. Wnt receptor signaling Nibaldo C. Inestrosa

• Covers the increasingly appreciated cell biology field of G-protein-coupled receptors
• Includes both established and new technologies
• Contributed by experts in the field