G Protein-Coupled Receptor Screening Assays (2nd Ed., 2nd ed. 2021) Methods and Protocols Methods in Molecular Biology Series, Vol. 2268
Coordonnateurs : Martins Sofia Aires M., Prazeres Duarte Miguel F.
1. Isolation of Lipid Rafts by the Detergent and Non-Detergent Based Methods for Localization of GPCRs with Immunoblotting and Laser Scanning Confocal Microscopy
Peter Abdelmaseeh, Andrew C. Tiu, Selim Rozyyev, Laureano D. Asico, Pedro A. Jose, Van Anthony M. Villar
2. Detection of GPCR mRNA Expression in Primary Cells via qPCR, Microarrays, and RNA-Sequencing
Krishna Sriram, Cristina Salmerón, Anna Di Nardo, and Paul A. Insel
3. Construction of Recombinant Cell Lines for GPCR Expression
Philip J. Reeves
4. Recombinant Expression and Purification of Cannabinoid Receptor CB2, a G Protein-Coupled Receptor
Alexei A. Yeliseev
5. Screening for Serotonin Receptor 4 Agonists Using GPCR-Based Sensor in Yeast
Emily A. Yasi and Pamela Peralta-Yahya
6. Immobilization of Olfactory Receptors Carried by Nanosomes onto a Gold Sensor Surface
Jasmina Vidic and Yanxia Hou
7. Screening Methods for Cell-Free Synthesized GPCR/Nanoparticle Samples
Zoe Köck, Volker Dötsch, and Frank Bernhard
8. Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs: The Case of Cy3B-Labelled Ligands Binding to MC4 Receptors in Budded Baculoviruses
Santa Veiksina, Maris-Johanna Tahk, Tõnis Laasfeld, Reet Link, Sergei Kopanchuk, and Ago Rinken
9. Bioluminescence in G Protein-Coupled Receptors Drug Screening Using Nanoluciferase and Halo-Tag Technology
Hannes Schihada, Katarina Nemec, Martin J. Lohse, and Isabella Maiellaro
10. Nanoluciferase-Based Complementation Assay to Detect GPCR-G Protein Interaction
Céline Laschet and Julien Hanson
11. Imaging of Genetically-Encoded FRET-Based Biosensors to Detect GPCR Activity
Luca Bordes, Sergei Chavez-Abiega, and Joachim Goedhart
12. cAMP Biosensor Assay Using BacMam Expression System: Studying the Downstream Signaling of LH/hCG Receptor Activation
Darja Lavogina, Tõnis Laasfeld, Maris-Johanna Tahk, Olga Kukk, Anni Allikalt, Sergei Kopanchuk, and Ago Rinken
13. FLIPR Calcium Mobilization Assays in GPCR Drug Discovery
Grzegorz Woszczek, Elisabeth Fuerst, and Thomas J.A. Maguire
14. Live Cell Imaging and Optogenetics-Based Assays for GPCR Activity
Xenia Meshik and N. Gautam
15. Split-Tobacco Etch Virus (Split-TEV) Method in G Protein-Coupled Receptor Interacting Proteins
Marta Alonso-Gardón and Raúl Estévez
16. NanoLuc-Based Methods to Measure β-Arrestin2 Recruitment to G Protein-Coupled Receptors
Xiaoyuan Ma, Rob Leurs, and Henry F. Vischer
17. Luciferase Complementation Approaches to Measure GPCR Signaling Kinetics and Bias
Nicola C. Dijon, Desislava N. Nesheva, and Nicholas D. Holliday
18. Gradient Tracking by Yeast GPCRs in a Microfluidics Chamber
Sara Kimiko Suzuki, Joshua B. Kelley, Timothy C. Elston, and Henrik G. Dohlman
19. Monitoring Intracellular Calcium in Response to GPCR Activation: Comparison Between Microtiter Plates and Microfluidic Assays
Sofia Aires M. Martins, Duarte Miguel F. Prazeres, Virginia Chu, and João P. Conde
20. Homology Modeling Using GPCRM Web Service
Przemysław Miszta, Szymon Niewieczerzał, Paweł Pasznik, and Sławomir Filipek
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Date de parution : 06-2022
Ouvrage de 327 p.
17.8x25.4 cm
Date de parution : 06-2021
Ouvrage de 327 p.
17.8x25.4 cm