Functional Proteomics, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1871

This book seeks to fill in the current technology gap with a specific collection of technologies developed for the study of protein function at a proteome scale. Chapters explore topics from protein functions to other aspects of protein analysis, especially in post-translational modification, as most proteomes use this mechanism in some capacity to carry out their unique role in cellular regulation. By comparing functional proteomes, this presents a bridge to other levels of system biology research including genomics and metabolomics in order to provide readers with a relatively complete picture for how one might study the biological system of their interest. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Functional Proteomics: Methods and Protocols collects these novel technologies in the hope that new frontiers in biological research will be created, important drug targets can be identified, and clinically validated biomarkers and diagnostic tests can be further developed.

Chapter-1: Current Landscape in System Biology and Functional Proteomics.

Chapter-2: Making the Case for Functional Proteomics

Chapter-3: Technology Overview

Chapter-4: Sample Collection and Processing (protocol)

Chapter-5: Serum Protein Enrichment (protocol)

Chapter-6: Gel-based Technologies (protocol)

Chapter-7: PEP Platform (protocol)

Chapter-8: Mass Spectrometry-based Technologies

Chapter-9: Chemical Proteomics (protocol)

Chapter-10: Computational methods for Functional Proteomics (protocol)

Chapter-11: Technologies on the Horizon

Chapter-12: Protein Post-translational Modification and Proteomics (protocol)

Chapter-13: Protein-Protein Interactions and Proteomics (protocol)

Chapter-14: Multi-functional Proteins and Proteomics (protocol)

Chapter-15: Functional Proteomics for Drug Target Identification and Validation (protocol)

Chapter-16: Functional Proteomics for Drug Safety Studies (protocol)

Chapter-17: Functional Proteomics for Drug Efficacy Studies (protocol)

Chapter-18: Biomarker Discovery from Serum (protocol)

Chapter-19: Biomarker Discovery during Drug Development (protocol)

Chapter-20: Functional Biomarkers (protocol)

Chapter-21: Proteomics in Biotechnology and Biologics Development (protocol)

Chapter-22: Proteomics in Ag-Biotechnology (protocol)

Chapter-23: Proteomics in Food Industry (protocol)

Chapter-24: Proteomics and Diseases

Chapter-25: Functional Proteomics in Perspective

Includes cutting-edge techniques for the study of functional proteomics

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts