ERK Signaling, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1487
Coordonnateur : Jimenez Gerardo
This volume provides a collection of techniques and approaches for the study of ERK signaling. It begins with a historical perspective of genetic and molecular discoveries, followed by chapters covering specific topics in a broad range of experimental systems, including in vitro assays of EGFR and ERK activities; proteomic and genome-wide analyses of ERK signaling targets; cell biological, genetic, quantitative and imaging approaches in cells and model organisms; and integrated mathematical modeling of the ERK pathway. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, ERK Signaling: Methods and Protocols will aid and stimulate further advances in the vibrant field of ERK signaling.
1. How Genetics has Helped Piece Together the MAPK Signaling Pathway
Dariel Ashton-Beaucage and Marc Therrien
2. In Vitro Enzyme Kinetics Analysis of EGFR
Zhihong Wang and Christine Candelora
3. High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells
4. Structural Studies of ERK2 Protein Complexes
5. Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk
6. Assaying Activation and Subcellular Localization of ERK in Cells and Tissues
Carme Caelles, Carles Bayod, and Melisa Morcillo
7. Detection and Functional Analysis of SUMO-Modified MEK
8. Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag
9. High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates
10. Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE
11. Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation
12. Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions
13. The Nuclear Translocation of ERK
14. Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA)
Zijian Tang and Chengkai Dai
15. Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors
16. Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers
17. Co-Culture Activation of MAP Kinase in Drosophila S2 Cells
18. Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling
19. 3D Organotypic Culture Model to Study Components of ERK Signaling
20. Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway
21. Genome-Wide Analysis of RAS/ERK Signaling Targets
22. Probing Chromatin Modifications in Response to ERK Signaling
23. Analyzing pERK Activation during Planarian Regeneration
24. Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development
25. Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images
26.
28. Modeling RASopathies with Genetically Modified Mouse Models
29. Dissecting Cell-Fate Determination through Integrated Mathematical Modeling of the ERK/MAPK Signaling Pathway
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains keynotes and implementation advice from the experts
Date de parution : 12-2016
Ouvrage de 440 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 105,49 €
Ajouter au panierDate de parution : 07-2018
Ouvrage de 440 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 116,04 €
Ajouter au panier