Epithelial Cell Culture, Softcover reprint of the original 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1817

Langue : Anglais

Coordonnateur : Baratta Mario

Couverture de l’ouvrage Epithelial Cell Culture

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This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from different organs, gastrointestinal system, thyroid, salivary gland, ovary, mammary gland, and olfactory epithelial tissue. Cell culture is a fundamental technique in both medical research and drug discovery and two-dimensional (2D) culture has been the preferred method, due to the ease with which cell monolayers can be induced to proliferate on planar surfaces. The book propose several functional assay useful to test cell activities. Further, The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures. Today, 3D cell cultures are emerging not only as a new tool in early drug discovery, but also as potential therapeutics to treat disease. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab.

1. Murine and human cancer mammary cell lines: functional tests

2. Bovine mammary organoids: a model to study epithelial mammary cells

3. Equine primary granulosa cell culture: analyses of transcriptome

4. Amniotic epithelial cells culture

5. Models of the small and large Intestinal epithelium: epithelial cell coculture

6. Differentiation of lung alveolar epithelial cells In Vitro

7. GnRH receptor analyses in pituitary αT3-1 cell line

8. Normal human thyrocytes in culture

9. Pig blastocyst culture: evaluation of maturation and steroid production

10. Primary pituitary cell culture in goldfish: hormonal analyses

11. Gastric Organoids: An Emerging Model System

12. Olfactory epithelium culture

13. Characterization of a coculture system of equine endometrial epithelial and stromal cells

14. Bovine endometrial epithelial cells analysis in protein expression

15. 3D Culture of pancreatic cells on decellularized Pancreatic bio-Scaffold

16. (3D) in vitro model replicating the intestinal functioning to dissect the stromal-epithelial interactions and evaluate the permeation of a model drug

17. Gastrospheres of human gastric mucosa cells: an in vitro model of stromal and epithelial stem cell niche reconstruction

18. Bovine lactotroph culture for the study of prolactin synthesis function

19. Granulosa cell coculture system to cultivate pig embryos

20. 3D primary epithelial cells culture system in mammary cell line

21. Mammospheres from murine mammary stem cell-enriched system

22. Three-dimensional cell culture model utilization in cancer stem cell research

23. Follicle-like model by granulosa cell encapsulation

24. Rat Pancreatic Islets in a Dynamic Culture

Mario Baratta is Full professor in Veterinary Physiology at the University of Turin (IT). He got the master degree in Veterinary Medicine at the University of Parma (IT) and the PhD in Neuroendocrinology of Animal Farm at the University of Bologna (IT). Today he is the Coordinator of the Graduate School in Veterinary Science for Animal Health and Food Safety, University of Turin. His main interests are the biology of adult stem cells in different tissues and he has developed specific knowledge in functional assessment systems of epithelial cells and cell differentiation in mammary gland and in muscle in animal science.

Includes cutting-edge methods and protocols for the study of epithelial cell culture

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts