Endocannabinoid Signaling, 1st ed. 2016 Methods and Protocols Methods in Molecular Biology Series, Vol. 1412

This volume encompasses all major methodologies to interrogate endocannabinoid systems (ECS) and endocannabinoids (eCBs) signaling. With increasing interest towards the manifold activities of eCBs, this book discusses the chemical, biochemical, and molecular biological assays, and activity of distinct elements of the ECS. These include membrane, nuclear receptors, biosynthetic and hydrolytic enzymes, and membrane transporters and oxidative enzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Timely and cutting edge, Endocannabinoid Signaling: Methods and Protocolsis a valuable resource and will help chemists, drug designers, biochemists, molecular biologists, cell biologists, pharmacologists, and (electro) physiologists navigate the mare magnum of endocannabinoid research.

1. Need for Methods to Investigate Endocannabinoid Signaling
Mauro Maccarrone

2. Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues
Laura Bindila and Beat Lutz

4. Analysis of Omega-3 Fatty Acid Derived N-acylethanolamines in Biological Matrices
Renger F. Witkamp and Michiel G.J. Balvers

5. Assay of CB₁ Receptor Binding
Valeria Catani and Valeria Gasperi

7.  Assay of TRPV1 Receptor Signaling<Aniello Schiano Moriello and Luciano De Petrocellis

8.  A Functional Assay for GPR55: Envision Protocols
Sharon Anavi-Goffer and Ruth A. Ross

9.  The Cyclic AMP Assay Using Human Cannabinoid CB₂ Receptor-Transfected Cells
Pietri Marini, Maria Grazia Cascio, and Roger G. Pertwee

11. Protocol to Study β-Arrestin Recruitment by CB₁ and CB₂Cannabinoid Receptors
Marjolein Soethoudt, Noortje van Gils, Mario van der Stelt, and Laura H. Heitman

12. Assay of NAT Activity
Toru Uyama and Matsuo Ueda

14. Assay of FAAH Activity
Monica Bari, Monica Feole, and Mauro Maccarrone

15. Assay of NAAA Activity
Kazuhito Tsuboi and Natsuo Ueda

16. Assay of DAGLα/β Activity
Tiziana Bisogno

17. Assay of Monoacylglycerol Lipase Activity
Kwang-Mook Jung and Daniele Piomelli

18. A Sensitive and Versatile Fluorescent Activity Assay for ABHD6
Juha R. Savinainen, Dina Navia-Paldanius, and Jarmo T. Laitinen

19. A Sensitive and Versatile Fluorescent Activity Assay for ABHD12
Juha R. Savinainen, Dina Navia-Paldanius, and Jarmo T. Laitinen
       

21. Assay of Endocannabinoid Oxidation by Cyclooxygenase-2
Shalley N. Kudalkar, Philip J. Kingsley, and Lawrence J. Marnett

22. Oxygenation of Anandamide by Lipoxygenases
Guus van Zadelhoff and Mario van der Stelt

23. Assay of Endocannabinoid Oxidation by Cytochrome P₄₅₀
Natasha T. Snider, Vyvyca J. Walker, and Paul F. Hollenberg

24. Assessing Gene Expression of the Endocannabinoid System
Mariangela Pucci and Claudio D’Addario

25. Western Blotting of the Endocannabinoid System
Jim Wager-Miller and Ken Kackie

26. Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells
Jim Wager-Miller and Ken Mackie

27. Measuring ECS Interaction with Biomembranes
Clotilde B. Angelucci, Annalaura Sabatucci, and Enrico Dainese

28. Visualization of Endocannabinoids in the Cell
Sergio Oddi, Antonio Totaro, and Mauro Maccarrone

Endocannabinoid Signaling: Methods and Protocols is a valuable resource and will help chemists, drug designers, biochemists, molecular biologists, cell biologists, pharmacologists, and (electro) physiologists navigate the mare magnum of endocannabinoid research.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implelementation advice from the experts