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Cryopreservation of Mammalian Gametes and Embryos, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1568

Langue : Anglais
Couverture de l’ouvrage Cryopreservation of Mammalian Gametes and Embryos

This volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations. The chapters in this book are divided into five parts and cover subjects such as: immature oocyte cryopreservation, human sperm vitrification and slow-freezing, directional freezing of ovarian tissue, automated vitrification systems, and detailed protocols on popular and commercially available cryopreservation/vitrification systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and practical, Cryopreservation of Mammalian Gametes and Embryos: Methods and Protocols includes chapters written by leading experts in the field and is a valuable resource for anyone interested in the field of cryopreservation. 

Preface
Table of Contents
Contributing Authors


Part I. Introduction

1. Historical Background on Gamete and Embryo Cryopreservation
Jaffar Ali, Naif H AlHarbi, and Nafisa Ali

Part II. Basics and Advanced Biology

2. Utility of Animal Models for Human Ovarian Tissue Cryopreservation
Seul Ki Kim, Jung Ryeol Lee, and S.Samuel Kim

3. Current Challenges in Immature Oocyte Cryopreservation
Ri-Cheng Chian, Yixin Xu, and Dana Keilty

4. Role of Antioxidants and Antifreeze Proteins in Cryopreservation/Vitrification
Seul Ki Kim, Hye Won Youm, Jung Ryeol Lee, and Chang Suk Suh

Part III. Methodology

5. Slow Freezing of Human Sperm
Ashok Agarwal and Eva Tvrda

6. Technology of Aseptic Cryoprotectant-free Vitrification of Human ICSI Spermatozoa
Vladimir Isachenko, Raul Sanchez, Peter Mallmann, Gohar Rahimi, and Evgenia Isachenko

7. Human Epididymal and Testicular Sperm Cryopreservation
Pankaj Talwar and Sarabpreet Singh

8. Human Oocytes Slow-rate Freezing: Methodology
C. Zacà and A. Borini

9. Slow Freezing and Thawing of Human Cleavage Stage Embryos
David H. Edgar, Janell Archer, and Debra A. Gook

10. Human Oocyte Vitrification
Laura Rienzi, Ana Cobo, and Filippo Maria Ubaldi

11. Human Embryo Vitrification
Juergen Liebermann

12. Human Ovarian Tissue Slow Freezing
Debra A. Gook

13. Human Ovarian Tissue Vitrification
Sherman Silber

14. Establishing an Oocyte Cryo Bank Network
James Graham, Josh Lim, and Michael Tucker

15. Development of a Nationwide Network for Ovarian Tissue Crypreservation
Jana Liebenthron and Markus Montag

Part IV. Advances in Cryotechnology, Research, and Animal Models

16. Directional Freezing of Ovarian Tissue and Freeze-drying of Stem Cells for Fertility Preservation
Amir Arav and Natan Yehudit

17. Sterile Plate-Based Vitrification of Adherent Human Pluripotent Stem Cells and Their Derivatives Using the TWIST Method
Julia C. Neubauer, Frank Stracke, and Heiko Zimmermann

18. Vitrification: A Reliable Method for Cryopreservation of Animal Embryos
B. Singh, G. Mal, and S. K. Singla

19. Cryopreservation Effect on Genetic Function: Neonatal Outcomes
Vanesa Robles, Marta F. Riesco, and David G. Valcarce

20. Gavi-Automated Vitrification Instrument
Tammie K. Roy, Susanna Brandi, and Teija Peura

Part V. Appendices

A. Cryotec® Vitrification Thawing
Goral Gandhi, Masashige Kuwayama, Sakina Kagalwala, and Priyanka Pangerkar

B. Solid Surface Vitrification
Mohan S. Kamath and Muthukumar K.

C. Automated Vitrification of Mammalian Embryos on a Digital Microfluidic Device
Jun Liu, Derek G. Pyne, Mohamed Abdelgawad, and Yu Sun

D. Irvine Scientific®  Vitrification System
Matthew VerMilyea and Amber Brewer

E. Rapid-i: Closed Vitrification Device by Vitrolife
Mark Larman

F. Quinn’s Advantage Embryo Freeze Kit
Kiri Beilby andPatrick Quinn

G. Vitrification of Blastocysts Using VitriBlast™ and ThermoBlast™ – Nidacon
Anna Niläng Laessker, Thorir Hardarsson, Ann-Sofie Forsberg, Tetsunori Mukaida, and Paul V. Holmes
Dr. Ashok Agarwal is the Head of the Clinical Andrology Center, which over the years under his leadership, has become a center of excellence in the United States for the diagnosis of male infertility and fertility preservation of men with oncological conditions. He is board certified as a Clinical Laboratory Director in Andrology by the American Board of Bioanalysis and an Inspector for the College of American Pathologists “Reproductive Laboratory Program” for accreditation of Andrology & IVF Laboratories. Dr. Agarwal is the recipient of several awards, such as the 2011 Innovator Award from Cleveland Clinic Innovations for the development of “Remote Sperm Banking Kits.” His current research interests are identifying biological markers of oxidative stress, DNA damage, and apoptosis using proteomic research tools and bioinformatics analysis, as well as preserving fertility in patients with cancer.

Zsolt Peter Nagy, M.D., Ph.D., is the Scientific Director at Reproductive Biology Associates (RBA), in Atlanta, GA, USA. He has acquired distinctive knowledge and experience on embryo science. He is well known for his contributions to oocyte vitrification and the development of the largest donor oocyte cryo-bank in North America. Dr. Nagy is a member/officer of several national and international professional societies, including ASRM, ESHRE, Alpha, ISFP and AFP, as well as a reviewer and editor. He is the author and/or co-author of over 200 publications, book chapters, and books.

Dr. Alex C. Varghese is the Scientific and Laboratory Director at Astra Fertility group in Mississauga, Canada. His proficiency and vast experience in clinical embryology and academics earned him a reputation in the ART community. He is well regarded for his passion to disseminate knowledge through online platforms and also as a leader of embryology society. He has helped many units establish training programs and af

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras

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