Correlative Light and Electron Microscopy IV
1. Workflow (dis)-advantages / spiderwebOri Avinoam2. Serial section LM + EMErin M. Tranfield3. Platinum clusters as CLEM probesPaul Verkade4. Correlative Light Electron Microscopy with a transition metal complex as a single probePaul Verkade5. Detection EDX + EELSBen N. G. Giepmans6. SEM-TEM-SIMSLouise Jensen7. HPF-CLEMXavier Heiligenstein8. A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyesThomas Müller-Reichert9. Three-dimensional on-section correlative light and electron microscopy of large cellular volumes using STEM tomographyKorbinian Buerger10. Accelerated procedure for approaching and imaging of optically branded ROI in tissueNatalia V. Gounko11. Volume-CLEMKedar Narayan12. Cryo SOFI CLEMRainer Kaufman13. Cryo fluo after Cryo lamellaPetr Chlanda14. Super resolution CLEMSílvia Pujals15. FIB-SEM + softwareAllon Weiner16. Correlia, CLEM softwareMatthias Schmidt17. Correlated Multimodality Imaging beyond CLEM: From in-vivo Preclinical Imaging to ex-vivo MicroscopyAndreas Walter18. Community effortsPaul Verkade
This book is aimed at PhD students, Post-doctoral researchers, and imaging facility staff working in the life science research area with an interest in microscopy technologies and applying or aiming to apply Correlative Microscopy techniques to their research.
- Contains contributions from experts in the field
- Covers topics using nano-SIMS and EDX for CLEM
- Presents recent advances and currently applied correlative approaches
- Gives detailed protocols, allowing for the application of workflows in one’s own laboratory setting
- Covers CLEM approaches in the context of specific applications
- Aims to stimulate the use of new combinations of imaging modalities
Date de parution : 03-2021
Ouvrage de 456 p.
19x23.3 cm
Thème de Correlative Light and Electron Microscopy IV :
Mots-clés :
Amira; Bio-imaging; C; elegans; CLEM; Click-chemistry; ColorEM; Correlation; Correlative imaging; Correlative light and electron microscopy; Correlative Light and Electron Microscopy; Correlative light and electron microscopy (CLEM); Correlative microscopy; Correlia; cryo-CLEM; Cryo-electron microscopy; cryo-EM; cryo-ET; Cryo-fluorescence microscopy; Cryogenic microscopy; Cryo-light microscopy; Cryo-LM; Data deposition; Deformable registration; EDS; EDX; Electron microscopy; EM; Endocytosis; FIB/SEM; Fibroblast growth factor receptor 1 (FGFR1); FIB-SEM; Finder dishes; Fluorescence microscopy; Fluorescently labeled gold nanoparticles; High-pressure freezing; Histology; HPF; Hyperplasia; Image archiving; Image processing; Image registration; ImageJ; ImageJ/Fiji; Imaging dishes; Invasion; Keyword3D-SEM; Live cell imaging; Luminescent metal complex; Micro-calcifications; Model organisms; Multimodal imaging; Multi-modal imaging; Multimodality imaging; Multimodality imaging across scales; Near-infrared branding; Open source; Polar body; Post-acquisition correlation; Preclinical imaging; Primary cilium; Resolution; Retinal pigment epithelial (RPE1) cells; Retracing; ROI; Scanning transmission electron microscopy (STEM) tomography; Shigella; Single molecule localization microscopy; STochastic Optical Reconstruction Microscopy (STORM); Super-resolution cryo-FM; Super-resolution microscopy; TEM; The microscopist's dilemma; Tissues; Tokuyasu cryo-sectioning; Training; Transmission electron microscopy; Vascular lesions; vEM; Vitrification; Volume electron microscopy; Volume EM
