Confocal Microscopy for Biologists, Softcover reprint of the original 1st ed. 2004

There has been a great upsurge in interest in light microscopy in recent years due to the advent of a number of significant advances in microscopy, one of the most important of which is confocal microscopy. Confocal microscopy has now become an important research tool, with a large number of new fluorescent dyes becoming available in the past few years, for probing your pet structure or molecule within fixed or living cell or tissue sampies. Many of the people interested in using confocal microscopy to further their research do not have a background in microscopy or even cell biology and so not only do they find considerable difficulty in obtaining satisfactory results with a confocal microscope, but they may be mislead by how data is being presented. This book is intended to teach you the basic concepts ofmicroscopy, fluorescence, digital imaging and the principles of confocal microscopy so that you may take full advantage ofthe excellent confocal microscopes now available. This book is also an excellent reference source for information related to confocal microscopy for both beginners and the more advanced users. For example, do you need to know the optimal pinhole size for a 63x 1. 4 NA lens? Do you need to know the fluorescence emission spectrum of Alexa 568? Access to the wealth of practical information in this book is made easier by using both the detailed index and the extensive glossary.

1. What Is Confocal Microscopy?.- 2. Understanding Microscopy.- 3. Confocal Microscopy Hardware.- 4. Image Collection.- 5. Digital Images In Microscopy.- 6. Imaging Software.- 7. Presentation And Publication.- 8. What Is Fluorescence?.- 9. Fluorescent Probes.- 10. Confocal Microscopy Techniques.- 11. Fluorescence Immunolabelling...- 12. Imaging Live Cells.- 13. The Internet.- 14. Technical Supplies.- 15. Further Reading.- Appendix 1: Confocal Microscopes.- Laser Spot Scanning Confocal Microscopes.- Nipkow Spinning Disk Confocal Microscopes.- Nipkow Disk / Micro-lens Array Confocal Microscopes.- A. Bio-Rad Cell Science Division.- Bio-Rad Instruments.- Radiance Series of Confocal Microscopes.- Components of the Radiance Confocal Microscopes.- Radiance Scan Head.- Radiance Instrument Control Unit (ICU).- Changing Optical Filters and Dichroic mirrors.- Radiance 2100 Rainbow.- Sels (Signal Enhancing Lens).- Photon Counting.- Computer and Software.- Mrc Series of Confocal Microscopes.- Components of the Mrc-1024 Confocal Microscope.- Mrc-1024 Scan Head.- Scan Head Alignment.- Optical Filter Blocks.- Emission Filter Wheels.- Photomultiplier Tubes (PMT).- Controller Box.- Computer Requirements.- Hardware set up for specific imaging methods.- Dual and Triple labelling — T 1 and T2a Filter Blocks.- Live Cell Work — B1 and Open Block Filter Blocks.- Back Scatter Imaging — B1 and T 1 Filter Blocks.- Trouble Shooting (Mrc And Radiance).- Bio-Rad Lasersharp Software.- Image Collection Panel.- Main Control Panel.- Optics Control Panel.- Mixer Control Panel.- Carl Zeiss Microscopy.- Zeiss Confocal Microscopes.- Zeiss Lsm 510 Meta Confocal Microscope.- Components of the Zeiss 510 Meta.- Zeiss Lsm 510 Meta Scan Head.- Lasers.- Single Track and Multi Track Image Collection.-Conventional Detection Channels.- Meta Channel.- Transmission Imaging.- Zeiss Confocal Microscope Control Software.- Main Control Panel.- Setting un the Microscope and Imaging Channels.- vSetting the Scan Control Parameters.- Adjusting Imagine Channel Settings.- Image Collection Panel.- Using the Meta Channel.- Meta Channel as a multi-Band Pass Optical Filter.- Meta Channel — Lambda Mode (Spectral Imaging).- Extracting Image Channels from a Spectral Stack.- Emission Fingerprinting.- On-Line Emission Fingerprinting.- Leica Microsystems.- Leica Confocal Microscopes.- Components of the Leica Spectral Imaging Instrument.- Leica Tcs Sp2 Confocal Microscope.- Leica “Ics Sp2 Scan Head.- Acoustic Optical Beam Splitter (AOBS).- Spectral Separation.- Uv Excitation.- Multi-Photon Excitation.- Transmission (DIC) Imaging.- Leica Confocal Microscope Control Software.- Main Control Panel — Monitor 1.- Changing the Desktop Knob Functions..- “Beam Path Setting” Control Panel.- Control “Buttons” for Image Collection.- Image Collection Panel — Monitor 2.- Nikon Instruments.- Nikon Confocal Microscopes.- Components of the Nikon C 1 Confocal Microscope.- Nikon C1 Scan Head.- Optics/Detector Unit.- Laser Launch Unit.- Nikon C1 User Interface.- Olympus Corporation.- Olympus Confocal Microscopes.- Components of the Olympus Fluoview 1000.- Fluoview 1000 Confocal Microscope.- Sim (Simultaneous) Scanner.- Fluoview 500 Confocal Microscope.- Fluoview 500 Scan Head.- Components of the Olympus Fluoview 300 and 500.- Fluoview 300 Confocal Microscope.- Fluoview 300 Scan Head.- Fluoview Software.- Image Collection Panel.- Main Control Panel.- Instrument settings Tabs.- Atto Bioscience.- Atto Bioscience Confocal Microscopes.- Carv Confocal Microscopes.- Components of the Carv ContocalMicroscope.- Carv Scan Head.- Yokogawa Electric Corporation.- CSU10 Scan Head.- CSU21 Scan Head.- Perkinelmer Life Sciences.- Perkinelmer Confocal Microscopes.- Ultra View Lc1 layout.- Components of the PerkinElmer Ultra View.- Visitech International.- Visitech Confocal Microscopes.

Explores the basic concepts of microscopy, fluorescence, digital imaging Outlines the principles of confocal microscopy Teaches readerds to take full advantage of the confocal microscopes now available