Chemical Proteomics, 2012 Methods and Protocols Methods in Molecular Biology Series, Vol. 803
Coordonnateurs : Drewes Gerard, Bantscheff Marcus
Authoritative and practical, Chemical Proteomics : Methods and Protocols seeks to provide methodologies that will contribute to a wider application of chemical proteomics methods in biochemical and cell biological laboratories.
Mass Spectrometry-Based Chemoproteomic Approaches.-Chemical Proteomics in Drug Discovery.-Compound Immobilization and Drug-Affinity Chromatography.-Affinity-based Chemoproteomics with Small Molecule-Peptide Conjugates.- A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine.-Probing Proteomes with Benzophenone Photoprobes.-Biotinylated Probes for the Analysis of Protein Modification by Electrophiles.-Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture Compound Mass Spectrometry (CCMS).-Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC.-Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric Mass Tags.-Affinity-Based Profiling of Dehydrogenase Subproteomes.- Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics.- Fluorescence-Based Proteasome Activity Profiling.- Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions.- Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDI Mass Spectrometry.- Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies.-Ligand Discovery Using Small-Molecule Microarrays.-Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of Kinetic Solubility.-A Database for Chemical Proteomics - ChEBI.-Working with Small Molecules: Rules-of-Thumb of “Drug Likeness”
Date de parution : 08-2016
Ouvrage de 313 p.
17.8x25.4 cm
Date de parution : 11-2011
Ouvrage de 313 p.
17.8x25.4 cm