Cell Migration, 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1749

This volume covers various assays and techniques that have been developed to study and characterize the cell migration in vitro, ex vivo, and in vivo. The chapters in this book present readers with the latest protocols to observe, quantify, and control cell migration. Some of the topics explored in this book are: migration in confined environments, microfluidic devices, optogenetics, chemotaxis, electrotaxis, detection of migrasomes, migration of Q cells in Caenorhabditis elegans, of Drosophila macrophages, optogenetics of cell migration, intravital imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and comprehensive, Cell Migration: Methods and Protocols is a valuable resource for anyone interested in learning more about this expanding field.

Preface…

Table of Contents…

Contributing Authors…

1. Random Migration Assays of Mammalian Cells and Quantitative Analyses of Single Cell Trajectories

Irene Dang and Alexis Gautreau

Nicolas Molinie and Alexis Gautreau

3. An In Vitro System to Study the Mesenchymal-to-Amoeboid Transition Aleksandra S. Chikina and Antonina Y. Alexandrova

4. An In Vitro System to Study the Epithelial-Mesenchymal Transition In Vitro Natalya A. Glushankova, Svetlana N. Rubtsova, and Irina Y. Zhitniak

5. Detection of Migrasomes

Yang Chen, Ying Li, Liang Ma, and Li Yu

6. 3D Endothelial Cell Migration

Kathryn A. Jacobs and Julie Gavard

7. Transmigration of Leukocytes across Epithelial Monolayers

Penny E. Morton and Maddy Parsons

8. Evaluation of Tumor Cell Invasiveness In Vivo: The Chick Chorioallantoic Membrane Assay

Selma Maacha and Simon Saule

9. Analysis of Invasion Dynamics of Matrix-Embedded Cells in a Multi-Sample Format

Marleen Van Troys, Paola Masuzzo, Lynn Huyck, Karima Bakkali, Davy Waterschoot, Lennart Martens, and Christophe Ampe

10. Using Systems Microscopy to Understand the Emergence of Cell Migration from Cell Organization

Staffan Strömblad and John G. Lock

11. Neuronal Precursor Migration in Ex Vivo Brain Slice Culture

Fu-Sheng Chou and Pei-Shan Wang

12. In Vitro Models to Analyze the Migration of MGE-Derived Interneurons

Claire Leclech and Christine Métin

13. Cell Migration in Tissues: Explant Culture and Live Imaging

Ralitza Staneva, Jorge Barbazan, Anthony Simon, Danijela Matic Vignjevic, and Denis Krndija

14. Intravital Imaging of Tumor Cell Motility in the Tumor Microenvironment Context

Battuya Bayarmagnai, Louisiane Perrin, Kamyar Esmaeilli Pourfarhangi, and Bojana Gligorijevic

15. Using the Zebrafish Embryo to Dissect the Early Steps of Metastasis Cascade Gautier Follain, Naël Osmani, Cédric Fuchs, Guillaume Allio, Sébastien Harlepp, and Jacky G. Goetz

16. Analysis of In Vivo Cell Migration in Mosaic Zebrafish Embryos

Arthur Boutillon, Florence A. Giger, and Nicolas B. David

17. Analysis of Cell Shape and Cell Migration of Drosophila Macrophages In Vivo Marike Rüder, Benedikt M. Nagel, and Sven Bogdan

18. Migration of Q Cells in Caenorhabditis elegans

Yongping Chai, Zhiwen Zhu, and Guangshuo Ou 

19. Imaging the Molecular Machines that Power Cell Migration

Anika Steffen, Frieda Kage, and Klemens Rottner

20. A Biologist-Friendly Method to Analyze Cross-Correlation between Protrusion Dynamics and Membrane Recruitment of Actin Regulators

Perrine Paul-Gilloteaux, François Waharte, Manish Kumar Singh, and Maria-Carla Parrini

Thomas Orré, Amine Mehidi, Sophie Massou, Olivier Rossier, and Grégory Giannone

22. Optogenetics of Cell Migration

Xenia Meshik, Patrick R. O’Neill, and N. Gautam

23. Electrotaxis: Cell Directional Movement in Electric Fields

Jolanta Sroka, Eliza Zimolag, Slawomir Lasota, Wlodzimierz Korohoda, and Zbigniew Madeja

24. Analysis of Random Migration of Dictyostelium Amoeba in Confined or Unconfined Environments

Christof Litschko, Julia Damiano-Guerico, Stefan Brühmann, and Jan Faix

Xiao Wang and Daniel Irimia

26. Leukocyte Migration and Deformation in Collagen Gels and Micro-Fabricated Constrictions

Pablo J. Sáez, Lucie Barbier, Rafaele Attia, Hawa-Racine Thiam, Matthieu Piel, and Pablo Vargas

27. Microfluidic Devices for Examining the Physical Limits of Migration in Confined Environments

Majid Malboubi, Asier Jayo, Maddy Parsons and Guillaume Charras

28. Controlling Confinement and Topology to Study Collective Cell Behaviors Guillaume Duclos, Maxime Deforet, Hannah G. Yevick, Olivier Cochet-Escartin, Flora Ascione, Sarah Moitrier, Trinish Sarkar, Victor Yashunsky, Isabelle Bonnet, Axel Buguin, and Pascal Silberzan

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts