Cell-free protein synthesis - methods and protocols Methods and Protocols

With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.

Introduction to Cell-free protein synthesis. The constructive approach for cell-free translation. Recombinant human proteins: comparative expression in vivo and in vitro. High-throughput materialization of genetic information using the wheat germ cell-free protein synthesis system. Cell-free expression of integral membrane proteins for structural study. Cell-free production of membrane proteins in presence of detergents. Novel techniques using PCR and cell-free protein synthesis systems for combinatorial bioengineering. Gene cloning and expression in molecular colonies (partial contents).

Alexander Spirin is a Professor at the Moscow State University, and the director of the Protein Research Institute of the Russian Academy of Sciences in Pushchino, Russia. He is one of the pioneers in the biochemical analysis of protein synthesis and has worked in the field for more than 40 years, performing the first-ever in vitro synthesis of proteins by using ribosome components. Professor Spirin has received many honors for his scientific achievements, including the Hans Adolf Krebs Medal, and is the author of several books on ribosomes and protein synthesis.

James Swartz is Professor of Chemical Engineering at Stanford University, California, USA. A graduate of the Massachusetts Institute of Technology, he has pioneered protein synthesis on an industrial scale while working for the companies Eli Lilly and Genentech. He is a leading expert on the biotechnological synthesis of mammalian proteins using bacterial whole cells and cell extracts.