Cell Cycle Synchronization (2nd Ed., 2nd ed. 2017) Methods and Protocols Methods in Molecular Biology Series, Vol. 1524
Coordonnateur : Banfalvi Gaspar
Practical and comprehensive, Cell Cycle Synchronization: Methods and Protocols, Second Edition is a valuable resource for PhD students and postdoctoral fellows, and researchers interested in general science, pharmacy, medicine and public health, computer science, and life sciences. Specialists and professionals in cell biology, genetics, molecular biology, biochemistry, and pharmacology will also find this book useful.
Preface...
Table of Contents...
Contributors...
Part I Cell Cycle Synchronization
1. Overview of Cell Synchronization
Gaspar Banfalvi
Part II Physical Fractionation
2. Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation
Gaspar Banfalvi
6. Synchronization of Mammalian Cell Cultures by Serum Deprivation
Thomas J. Langan, Kyle R. Rodgers, and Richard C. Chou
7. DNA Damage Response Resulting from Replication Stress Induced by Synchronization of Cells by Inhibitors of DNA Replication: Analysis by Flow Cytometry
H. Dorota Halicka, Hong Zhao , Jiangwei Li, Jorge Garcia, Monika Podhorecka, and Zbigniew Darzynkiewicz
8. Chromosome Formation During Fertilization in Eggs of the Teleost Oryzias Latipes
Takashi Iwamatsu
9. Flow Cytometry Analysis of Cell Cycle and Specific Cell Synchronization with Butyrate
Cong-Jun Li
10. Chemically Induced Cell Cycle Arrest in Perfusion Cell Culture
Gabor Nagy, Bence Tanczos, Eszter Fidrus, Laszlo Talas and Gaspar Banfalvi
11. Analysis of Nuclear Uracil DNA – Glycosylase (nUDG) Turnover During the Cell Cycle
Jennifer A. Fischer and Salvatore Caradonna
12. Synchronization of Hela Cells
Hoi Tang Ma and Randy Y.C. Poon
Part IV Synchronization of Unicellular Organisms
13. Synchronization of Bacillus Subtilis Cells by Spore Germination and Outgrowth
Gaspar Banfalvi
14. Synchronization of Yeast
Jessica Smith, Arkadi Manukyan, Hui Hua, Huzefa Dungrawala, and Brandt L. Schneider
15. Synchronization of Pathogenic Protozoans
Staffan Swärd and Karin Troell
Part V Synchronizing Mammalian and Transfected Cells
16. Synchronization of In Vitro Maturation in Porcine Oocytes
Tamas Somfai and Yuhi Hirao
Part VI Synchronization of Plant Cells
17. Detection of Changes in the Medicago sativa Retinoblastoma-Related Protein (MsRBR1)
Phosphorylation during Cell Cycle Progression in Synchronized Cell Suspension Culture
Ferhan Ayaydin, Edit Kotogány, Edit Ábrahám, and Gábor V. Horváth
Part VII Synchronization of Embryonic Cells
18. Nuclear Treatment and Cell Cycle Synchronization for the Purpose of Mammalian and Primate Somatic Cell Nuclear Transfer (SCNT)
Yoel Sufaro and Benjamin E. Reubinoff
Part VIII Hematopoietic Stem Cells
19. Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation
Kap-Hyoun Ko, Robert Nordon, Tracey A. O’Brien, Geoff Symonds, and Alla Dolnikov
Part IX Clinical Study
20. Intracellular Flow Cytometry Improvements in Clinical Studies
Julie Demaret, Morgane Gossez, Fabienne Venet, and Guillaume Monneret
Part X Cell Cycle Control
21. Molecular Network Dynamics of Cell Cycle Control: Periodicity of Start and Finish
Alida Palmisano, Judit Zámborszky, Cihan Oguz, and Attila Csikász-Nagy
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Ouvrage de 354 p.
17.8x25.4 cm
Ouvrage de 354 p.
17.8x25.4 cm