Bacterial Pathogenesis, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1535
Coordonnateurs : Nordenfelt Pontus, Collin Mattias
This volume discusses various methods and protocols used for the experimentation of a wide range of bacterial species, such as Streptococcus pyogenes, Staphylococcus aureus, Streptococcus pneumonia, Listeria monocytogenes, and Mycobacterium marinum. Bacterial Pathogens: Methods and Protocols is divided into 6 parts: Part 1 describes different approaches to identifying and characterizing bacterial effector molecules; Part 2 deals with structural biology of bacterial pathogenesis and how to overcome folding and stability problems with recombinantly expressed proteins; Part 3 details methodology that identifies bacteria in complex communities and how genomes of bacterial pathogens have evolved; Part 4 reflects on the rapid development of advanced imaging techniques that address questions about molecular properties of individual live bacteria, ultrastructure of surfaces, and subcellular localization of bacterial proteins; Part 5 describes methods from in vitro and in vivo modeling of bacterial infections; and Part 6 explores how bacterial pathogens are the true experts of the immune system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Bacterial Pathogens: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.
Preface...
Table of Contents...
Contributing Authors...
Part I Identification and Characterization of Bacterial Effector Molecules
1. Protein-Based Strategies to Identify and Isolate Bacterial Virulence Factors
Rolf Lood and Inga-Maria Frick
2. Analysis of Bacterial Surface Interactions with Mass Spectrometry-Based Proteomics
Christofer Karlsson, Johan Teleman, and Johan Malmström
3. Differential Radial Capillary Action of Ligand Assay (DRaCALA) for High-Throughput Detection of Protein-Metabolite Interactions in Bacteria
Mona W. Orrand Vincent T. Lee
4. Identifying Bacterial Immune Evasion Proteins using Phage Display
Cindy Fevre, Lisette Scheepmaker, and Pieter-Jan Haas
Part II Structural Biology of Bacterial–Host Interactions
5. Competition for Iron between Host and Pathogen: A Structural Case Study on Helicobacter pylori
Wei Xia
6. Common Challenges in Studying the Structure and Function of Bacterial Proteins: Case Studies from Helicobacter pylori
Daniel A. Bonsor and Eric J. Sundberg
Part III Genetics and Phylogenetics of Bacterial Pathogens
7. Development of a Single Locus Sequence Typing (SLST) Scheme for Typing Bacterial Species Directly from Complex Communities
Christian F. P. Scholz and Anders Jensen
8. Reconstructing the Ancestral Relationships between Bacterial Pathogen Genomes
Caitlin Collins and Xavier Didelot
Part IV Bacterial Imaging Approaches and Related Techniques
9. Making Fluorescent Streptococci and Enterococci for Live Imaging
Sarah Shabayek and Barbara Spellerberg
10. Computer Vision-Based Image Analysis of Bacteria
Jonas Danielsen and Pontus Nordenfelt
11. Assessing Vacuolar Escape of Listeria Monocytogenes
Juan J. Quereda, Martin Sachse, Damien Balestrino, Théodore Grenier, Jennifer Fredlund, Anne Danckaert, Nathalie Aulner, Spencer Shorte, Jost Enninga, Pascale Cossart, and Javier Pizarro-Cerdá
12. Immobilization Techniques of Bacteria for Live Super-Resolution Imaging using Structured Illumination Microscopy
Ann L. Bottomley, Lynne Turnbull, Cynthia B. Whitchurch, and Elizabeth J. Harry
13. Negative Staining and Transmission Electron Microscopy of Bacterial Surface Structures
Matthias Mörgelin
14. Detection of Intracellular Proteins by High-Resolution Immunofluorescence Microscopy in Streptococcus pyogenes
Assaf Raz
15. Antibody Guided Molecular Imaging of Infective Endocarditis Infection
Kenneth L. Pinkston, Peng Gao, Kavindra V. Singh, Ali Azhdarinia, Barbara E. Murray, Eva M. Sevick-Muraca, and Barrett R. Harvey
Part V Models for Studying Bacterial Pathogenesis
16. The Zebrafish as a Model for Human Bacterial Infections
Melody N. Neely
17. Determining Platelet Activation and Aggregation in Response to Bacteria
Oonagh Shannon
18. Killing Bacteria with Cytotoxic Effector Proteins of Human Killer Immune Cells: Granzymes, Granulysin and Perforin
Diego López León, Isabelle Fellay, Pierre-Yves Mantel, and Michael Walch
19.In Vitro and In Vivo Biofilm Formation by Pathogenic Streptococci
Yashuan Chao, Caroline Bergenfelz, and Anders P. Håkansson
20. Murine Mycobacterium marinum Infection as a Model for Tuberculosis
Julia Lienard and Fredric Carlsson
Part VI Methods Exploiting Bacterial Immune Evasion
21. Generating and Purifying Fab Fragments from Human and Mouse IgG using the Bacterial Enzymes IdeS, SpeB and Kgp
Jonathan Sjögren, Linda Andersson, Malin Mejàre, and Fredrik Olsson
22. Measuring Antibody Orientation at the Bacterial Surface
Oonagh Shannon and Pontus Nordenfelt
23. Towards Clinical use of the IgG Specific Enzymes IdeS and EndoS against Antibody-Mediated Diseases
Mattias Collin and Lars Björck
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains keynotes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Ouvrage de 357 p.
17.8x25.4 cm
Ouvrage de 357 p.
17.8x25.4 cm
