Arbuscular Mycorrhizal Fungi, 1st ed. 2020 Methods and Protocols Methods in Molecular Biology Series, Vol. 2146

1. Isolation and Culture of Arbuscular Mycorrhizal Fungi from Field Samples

            Dora Trejo-Aguilar and Jacob Banuelos

2. The Mycorrhizal Donor Plant (MDP) In Vitro Culture System for the Efficient Colonization of Whole Plants

            Ismahen Lalaymia and Stéphane Declerck

3. A Whole-Plant Culture Method to Study Structural and Functional Traits of Extraradical Mycelium

            Cristiana Sbrana, Alessandra Pepe, Nuria Ferrol, and Manuela Giovannetti

4. Histochemical Staining and Quantification of Arbuscular Mycorrhizal Fungal Colonization

            Tania Ho-Plágaro, María Isabel Tamayo-Navarrete, and José Manuel García-Garrido

5. Fluorescent Staining of Arbuscular Mycorrhizal Structures Using Wheat Germ Agglutinin (WGA) and Propidium Iodide

            Gennaro Carotenuto and Andrea Genre

6. Visualization of Arbuscular Mycorrhizal Fungal Extra-Radical Hyphae and Spores Vitality and Activity

            Pablo Cornejo and Humberto Aponte

7. Molecular Quantification of Arbuscular Mycorrhizal Fungal Root Colonization

8. Single-Spore-Extraction for Genetic Analyses of Arbuscular Mycorrhizal Fungi

            Dirk Redecker

9. High Throughput DNA Sequence-Based Analysis of AMF Communities

            Íris Marisa Maxaieie Victorino, Andrea Berruti, Alberto Orgiazzi, Samuele Voyron, Valeria Bianciotto, and Erica Lumini

10. Analysis of Arbuscular Mycorrhizal Fungal Communities by Terminal Restriction Fragment Length Polymorphism (TRFLP)

            Álvaro López-García

11. Bioinformatic Methods for the Analysis of High-Throughput RNA Sequencing in Arbuscular Mycorrhizal Fungi

            Francesco Venice, Alessandra Salvioli di Fossalunga, and Paola Bonfante

12. Arbuscular Mycorrhizal Fungal Gene Expression Analysis by Real-Time PCR

            Alessandro Silvestri, Jacob Pérez-Tienda, and Juan Antonio López-Raez

13. Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis

            Raffaella Balestrini and Valentina Fiorilli

14. Detection of Arbuscular Mycorrhizal Fungal Gene Expression by In Situ Hybridization

            Bettina Hause and Natalia Requena

15. Functional Analyses of Arbuscular Mycorrhizal Fungal Genes in Yeast

16. Isotope Labelling to Study Phosphorus Uptake in the Arbuscular Mycorrhizal Symbiosis

            Carla Cruz-Paredes and Mayra E. Gavito

17. Recovery of Extra-Radical Fungal Peptides Amenable for Shotgun Protein Profiling in Arbuscular Mycorrhizae

            Ghislaine Recorbet, Pierre-Emmanuel Courty, and Daniel Wipf

18. Host-Induced Gene Silencing of Arbuscular Mycorrhizal Fungal Genes via Agrobacterium Rhizogenes-Mediated Root Transformation in Medicago truncatula

            Meike Hartmann, Stefanie Voß, and Natalia Requena

19. Application of Virus-Induced Gene Silencing to Arbuscular Mycorrhizal Fungi

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts