Antibiotics, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1520
Coordonnateur : Sass Peter
This volume provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, and methods for the analysis and detection of microbial adaptation strategies. Antibiotics: Methods and Protocols guides readers through chapters on production and design, mode of action, and response and susceptibility. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Antibiotics: Methods and Protocols aims to inspire scientific work in the exciting field of antibiotic research.
Part I: Production and Design
1. Antibiotics - Precious Goods in Changing Times
Peter Sass
2. Mining Bacterial Genomes for Secondary Metabolite Gene Clusters
Martina Adamek, Marius Spohn, Evi Stegmann, and Nadine Ziemert
3. Production of Antimicrobial Compounds by Fermentation
Henrik Harms, Gabriele M. König and Till F. Schäberle
4. Structure Elucidation of Antibiotics by NMR Spectroscopy
Georgios Daletos, Elena Ancheeva, Raha Orfali, Victor Wray, and Peter Proksch
5. Computer-Aided Drug Design Methods
Wenbo Yu and Alexander D. MacKerell, Jr.
6. Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents
Alexander Zippererand Dorothee Kretschmer
Part II: Mode of Action
7. Application of a Bacillus subtilis Whole-cell Biosensor (PliaI-lux)for the Identification of Cell Wall Active Antibacterial Compounds
Carolin Martina Kobras, Thorsten Mascher, and Susanne Gebhard
8. Determination of Bacterial Membrane Impairment by Antimicrobial Agents
Miriam Wilmes and Hans-Georg Sahl
9. Mass-sensitive Biosensor Systems to Determine the Membrane Interaction of Analytes
Sebastian G. Hoß and Gerd Bendas
10. Measurement of Cell Membrane Fluidity by Laurdan GP:Fluorescence Spectroscopy and Microscopy
Kathi Scheinpflug, Oxana Krylova and Henrik Strahl
11. In vitro Assays to Identify Antibiotics Targeting DNA Metabolism
Yu Chen, Xin Liu, Nancy Wu, and Carol A. Fierke
13. Reporter Gene-based Screening for TPP Riboswitch Activators
Christina E. Lünse and Günter Mayer
14. Cell-based Fluorescent Screen to Identify Inhibitors of Bacterial Translation InitiationFederica Briani
15. Bacterial Histidine Kinases: Overexpression, Purification, and Inhibitor Screen
Mike Gajdiss, Michael Türck, and Gabriele Bierbaum
Part III: Response and Susceptibility
16. Expression Profiling of Antibiotic Resistant Bacteria Obtained by Laboratory Evolution
Shingo Suzuki, Takaaki Horinouchi, and Chikara Furusawa 17. Sample Preparation for Mass-Spectrometry Based Absolute Protein Quantification in Antibiotic Stress ResearchFlorian Bonn, Sandra Maass, and Dörte Becher
18. Label-Free Quantitation of Ribosomal Proteins from Bacillus Subtillis for Antibiotic Research
Sina Schäkermann, Pascal Prochnow, and Julia E. Bandow
19. Functional Metagenomics to Study Antibiotic Resistance
Manish Boolchandani, Sanket Patel, and Gautam Dantas
20. Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains using High Throughput Sequencing
Miguel Paulo Machado, Bruno Ribeiro-Gonçalves, Mickael Silva, Mário Ramirez, João André Carriço
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Ouvrage de 358 p.
17.8x25.4 cm
Ouvrage de 358 p.
17.8x25.4 cm
