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Alpha-Synuclein, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1948

Langue : Anglais

Coordonnateur : Bartels Tim

Couverture de l’ouvrage Alpha-Synuclein

This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and functional deficits in cells and mice. Chapters also cover topics such as kinetic measurements of endocytosis and exocytosis in cultured neurons; electrochemiluminescence-based detection; yeast-based screens to target alpha-synuclein toxicity; mass-spectrometry of alpha-synuclein in rat cortical neurons; and expression and purification of untagged a-synuclein. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Alpha-Synuclein: Methods and Protocols is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects.


Preface…
Table of Contents…
Contributing Authors…
1. Assays for Neuronal Defects Caused by Early Formation of α-Synuclein Inclusions in Primary Cultured Neurons
Laura A. Volpicelli-Daley

2. Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue
John B. Sanderson

3. Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons
Karina Vargas

4. Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform
Luis Concha-Marambio, Mohammad Shahnawaz, and Claudio Soto

5. Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain using Pre-Formed Fibrils
Bin Zhang, Victoria Kehm, Ron Gathagan, Susan N. Leight, John Q. Trojanowski, Virginia M.-Y. Lee, and Kelvin C. Luk

6. Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection
Niels Kruse and Brit Möllenhauer

7. Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers
Rosalind F. Roberts, Nora Bengoa-Vergniory, and Javier Alegre-Abarrategui

8. Studying α-Synuclein Conformation by Intact-Cell Crosslinking
Thibaut Imberdis, Saranna Fanning, Andrew Newman, Nagendran Ramalingam, and Ulf Dettmer

9. C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques
Mandy Koopman, Renée I. Seinstra, and Ellen. A. A. Nollen

10. Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein
Tara A. Eastwood and Daniel P. Mulvihill

11. Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes
Matteo Rovere

12. Yeast-Based Screens to Target Alpha-Synuclein Toxicity
Inês Caldeira Brás, Blagovesta Popova, Gerhard H. Braus, and Tiago F. Outeiro

13. Probing Structural Changes in Alpha Synuclein by Nuclear Magnetic Resonance Spectroscopy
Tapojyoti Das and David Eliezer

14. Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers
Eric S. Luth and Irina G. Stavrovskaya

15. Monitoring α-Synuclein Proteotoxicity in Drosophila Models
Aron Szabo and George K. Tofaris

16. Detection of α-Synuclein in Biological Samples using Mass Spectrometry
Ann Brinkmalm, Annika Öhrfelt, Payel Bhattacharjee, and Henrik Zetterberg

17. Single-Molecule FRET Detection of Early-Stage Conformations in α-Synuclein Aggregation
Mahdi Muhammad Moosa, Josephine C. Ferreon, and Allan Chris M. Ferreon 

18. In Situ Peroxidase Labelling and Mass-Spectrometry of Alpha-Synuclein in Rat Cortical Neurons
Ricardo Sanz, Patrick Ovando-Roche, Namrata D. Udeshi, Steven A. Carr, Chee Yeun Chung, and Vikram Khurana

19. Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy
Julia Cattani, Theresa Braun, and Malte Drescher

20. Expression and Purification of Untagged α-Synuclein
Alex E. Powers and Dushyant S. Patel

21. Generation and Analysis of Viral Vector Mediated Rodent Models for Parkinson’s Disease
Akua A. Karikari, James B. Koprich, and Chi Wang Ip

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Ouvrage de 289 p.

17.8x25.4 cm

Sous réserve de disponibilité chez l'éditeur.

210,99 €

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