3D Cell Culture, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1612
Coordonnateur : Koledova Zuzana
Zuzana Koledova
Part I: Hydrogels and Scaffolds for 3D Cell Culture
<2. Preparation of Decellularized Biological Scaffolds for 3D Cell Culture
Bryan N. Brown, Michael J. Buckenmeyer, and Travis A. Prest
3. 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix
Katrina Wisdom and Ovijit Chaudhuri
4. Hydrogel-Based In Vitro Models of Tumor Angiogenesis
Laura J. Bray, Marcus Binner, Uwe Freudenberg, and Carsten Werner
5. Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional PEG Hydrogels
Massimiliano Caiazzo, Yoji Tabata, and Matthias Lutolf
6. Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modelling in Three-Dimensions
Edgar B. Montufar, Lucy Vojtova, Ladislav Celko, and Maria-Pau Ginebra
Part II: 3D Organoid and Organotypic Cultures
7. Establishment of 3D Intestinal Organoid Cultures from Intestinal Stem Cells
Shinya Sugimoto and Toshiro Sato
8. 3D Co-Culture of Mammary Organoids with Fibrospheres: A Model for Studying Epithelial-Stromal Interactions During Mammary Branching Morphogenesis
Zuzana Koledova
9. An Organotypic 3D Assay for Primary Human Mammary Epithelial Cells that Recapitulates Branching Morphogenesis
Jelena R. Linnemann, Lisa K. Meixner, Haruko Miura, and Christina H. Scheel
10. 3D Primary Culture Model to Study Human Mammary Development
Daniel H. Miller, Ethan S. Sokol, and Piyush B. Gupta
11. Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells
Anas Rabata, Ales Hampl, and Zuzana Koledova
12. 3D Hanging Drop Culture to Establish Prostate Cancer Organoids
Theresa Eder and Iris E. Eder
13. 3D Fluid-Dynamic Culture Models of Multiple Myeloma
Marina Ferrarini, Nathalie Steimberg, Jennifer Boniotti, Angiola Berenzi, Daniela Belloni, Giovanna Mazzoleni, and Elisabetta Ferrero
14. Preparation of a Three-Dimensional Full Thickness Skin Equivalent
Christian Reuter, Heike Walles, and Florian Groeber
15. Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System
Romana E. Ranftl and Fernando Calvo
16. 3D Co-Culture Model of the Brain Parenchyma/Metastasis Interface of Brain Metastasis
Raquel Blazquez and Tobias Pukrop
Part III: Micropatterning
17. 3D Neural Culture in Dual Hydrogel Systems
J. Lowry Curley and Michael J. Moore
18. 3D Cell Culture in Micro-Patterned Hydrogels Prepared by Photomask, Micro-Needle, or Soft Lithography Techniques
Seyedsina Moeinzadeh and Esmaiel Jabbari
19. 3D Stem Cell Niche Engineering Via Two-Photon Laser Polymerization
Michele M. Nava, Tommaso Zandrini, Giulio Cerullo, Roberto Osellame, and Manuela T. Raimondi
Part IV: Microfluidic Approaches for 3D Cell Culture
20. Microfluidic-Based Generation of 3D Collagen Spheres to Investigate Multicellular Spheroid Invasion
Fabien Bertillot, Youmna Attieh, Morgan Delarue, Basile G. Gurchenkov, Stephanie Descroix, Danijela Matic Vignjevic, and Davide Ferraro
21. High-Throughput Cancer Cell Sphere Formation for 3D Cell Culture
Yu-Chih Chen and Euisik Yoon
22. High-Throughput 3D Tumor Culture in a Recyclable Microfluidic Platform
Wenming Liu and Jinyi Wang
23. High-Throughput Microfluidic Platform for 3D Cultures of Mesenchymal Stem Cells
Paola Occhetta, Roberta Visone, and Marco Rasponi
24. 3D Anastomosed Microvascular Network Model with Living Capillary Networks and Endothelial Cell-Lined Microfluidic Channels
Xiaolin Wang, Duc T.T. Phan, Steven C. George, Christopher C.W. Hughes, and Abraham P. Lee
25. Human Lung Small Airway-on-a-Chip Protocol
Kambez H. Benam, Marc Mazur, Youngjae Choe, Thomas C. Ferrante, Richard Novak, and Donald E. Ingber
Part V: Bioprinting
26. Microfluidic Bioprinting of Heterogeneous 3D Tissue Constructs
Cristina Colosi, Marco Costantini, Andrea Barbetta, and Mariella Dentini
27. Bioprinting of 3D Tissue Models Using Decellularized Extracellular Matrix Bioink
Falguni Pati and Dong-Woo Cho
28. Bioprinting Cartilage Tissue from Mesenchymal Stem Cells and PEG Hydrogel
Guifang Gao, Karen Hubbell, Arndt F. Schilling, Guohao Dai, and Xiaofeng Cui
Part VI: Imaging and Image Analysis of 3D Cell Cultures
29. Real-Time Cell Cycle Imaging in a 3D Cell Culture Model of Melanoma
Loredana Spoerri, Kimberley A. Beaumont, Andrea Anfosso, and Nikolas K. Haass
30. Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy
Josef Jaros, Michal Petrov, Marketa Tesarova, and Ales Hampl
31. Quantitative Phenotypic Image Analysis of Three-Dimensional Organotypic Cultures
Malin Åkerfelt, Mervi Toriseva, and Matthias Nees
Date de parution : 06-2017
Ouvrage de 452 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 131,86 €
Ajouter au panier