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3D Cell Culture, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1612

Langue : Anglais

Coordonnateur : Koledova Zuzana

Couverture de l’ouvrage 3D Cell Culture
This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and cutting-edge, 3D Cell Culture: Methods and Protocols aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures.


Chapter 15 of this book is available open access under a CC BY 4.0 license.
1. 3D Cell Culture: An Introduction

            Zuzana Koledova

 

Part I: Hydrogels and Scaffolds for 3D Cell Culture

 

<2. Preparation of Decellularized Biological Scaffolds for 3D Cell Culture

            Bryan N. Brown, Michael J. Buckenmeyer, and Travis A. Prest

 

3. 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix

            Katrina Wisdom and Ovijit Chaudhuri

 

4. Hydrogel-Based In Vitro Models of Tumor Angiogenesis

            Laura J. Bray, Marcus Binner, Uwe Freudenberg, and Carsten Werner

 

5. Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional PEG Hydrogels

            Massimiliano Caiazzo, Yoji Tabata, and Matthias Lutolf

 

6. Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modelling in Three-Dimensions

            Edgar B. Montufar, Lucy Vojtova, Ladislav Celko, and Maria-Pau Ginebra

 

Part II: 3D Organoid and Organotypic Cultures

 

7. Establishment of 3D Intestinal Organoid Cultures from Intestinal Stem Cells

            Shinya Sugimoto and Toshiro Sato

 

8. 3D Co-Culture of Mammary Organoids with Fibrospheres: A Model for Studying Epithelial-Stromal Interactions During Mammary Branching Morphogenesis

            Zuzana Koledova

 

9. An Organotypic 3D Assay for Primary Human Mammary Epithelial Cells that Recapitulates Branching Morphogenesis

            Jelena R. Linnemann, Lisa K. Meixner, Haruko Miura, and Christina H. Scheel

 

10. 3D Primary Culture Model to Study Human Mammary Development

            Daniel H. Miller, Ethan S. Sokol, and Piyush B. Gupta

 

11. Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells

            Anas Rabata, Ales Hampl, and Zuzana Koledova

 

12. 3D Hanging Drop Culture to Establish Prostate Cancer Organoids

            Theresa Eder and Iris E. Eder

 

13. 3D Fluid-Dynamic Culture Models of Multiple Myeloma

            Marina Ferrarini, Nathalie Steimberg, Jennifer Boniotti, Angiola Berenzi, Daniela Belloni, Giovanna Mazzoleni, and Elisabetta Ferrero

 

14. Preparation of a Three-Dimensional Full Thickness Skin Equivalent

            Christian Reuter, Heike Walles, and Florian Groeber

 

15. Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System

            Romana E. Ranftl and Fernando Calvo

 

16. 3D Co-Culture Model of the Brain Parenchyma/Metastasis Interface of Brain Metastasis

            Raquel Blazquez and Tobias Pukrop

 

Part III: Micropatterning

 

17. 3D Neural Culture in Dual Hydrogel Systems

            J. Lowry Curley and Michael J. Moore

 

18. 3D Cell Culture in Micro-Patterned Hydrogels Prepared by Photomask, Micro-Needle, or Soft Lithography Techniques

            Seyedsina Moeinzadeh and Esmaiel Jabbari

 

19. 3D Stem Cell Niche Engineering Via Two-Photon Laser Polymerization

            Michele M. Nava, Tommaso Zandrini, Giulio Cerullo, Roberto Osellame, and Manuela T. Raimondi

 

Part IV: Microfluidic Approaches for 3D Cell Culture

 

20. Microfluidic-Based Generation of 3D Collagen Spheres to Investigate Multicellular Spheroid Invasion

            Fabien Bertillot, Youmna Attieh, Morgan Delarue, Basile G. Gurchenkov, Stephanie Descroix, Danijela Matic Vignjevic, and Davide Ferraro

 

21. High-Throughput Cancer Cell Sphere Formation for 3D Cell Culture

            Yu-Chih Chen and Euisik Yoon

 

22. High-Throughput 3D Tumor Culture in a Recyclable Microfluidic Platform

            Wenming Liu and Jinyi Wang

 

23. High-Throughput Microfluidic Platform for 3D Cultures of Mesenchymal Stem Cells

            Paola Occhetta, Roberta Visone, and Marco Rasponi

 

24. 3D Anastomosed Microvascular Network Model with Living Capillary Networks and Endothelial Cell-Lined Microfluidic Channels

            Xiaolin Wang, Duc T.T. Phan, Steven C. George, Christopher C.W. Hughes, and Abraham P. Lee

 

25. Human Lung Small Airway-on-a-Chip Protocol

            Kambez H. Benam, Marc Mazur, Youngjae Choe, Thomas C. Ferrante, Richard Novak, and Donald E. Ingber

 

Part V: Bioprinting

 

26. Microfluidic Bioprinting of Heterogeneous 3D Tissue Constructs

            Cristina Colosi, Marco Costantini, Andrea Barbetta, and Mariella Dentini

 

27. Bioprinting of 3D Tissue Models Using Decellularized Extracellular Matrix Bioink

            Falguni Pati and Dong-Woo Cho

 

28. Bioprinting Cartilage Tissue from Mesenchymal Stem Cells and PEG Hydrogel

            Guifang Gao, Karen Hubbell, Arndt F. Schilling, Guohao Dai, and Xiaofeng Cui

 

Part VI: Imaging and Image Analysis of 3D Cell Cultures

 

29. Real-Time Cell Cycle Imaging in a 3D Cell Culture Model of Melanoma

            Loredana Spoerri, Kimberley A. Beaumont, Andrea Anfosso, and Nikolas K. Haass

 

30. Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy

            Josef Jaros, Michal Petrov, Marketa Tesarova, and Ales Hampl

 

31. Quantitative Phenotypic Image Analysis of Three-Dimensional Organotypic Cultures

            Malin Åkerfelt, Mervi Toriseva, and Matthias Nees

Includes cutting-edge methods and protocols for the study of 3D cell cultures
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from experts

Date de parution :

Ouvrage de 452 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

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