Buckwheat, 1st ed. 2024 Methods and Protocols Methods in Molecular Biology Series, Vol. 2791

Callus Induction Followed by Regeneration and Hairy Root Induction in Common Buckwheat.- Immunostaining for Epigenetic Modifications in FagopyrumCalli.- Quantitative Analysis of Epigenetic Modifications in Fagopyrum Nuclei with Confocal Microscope, ImageJ, and R Studio.- Phenolic Determination in Proembryogenic Cell Complexes of Buckwheat Morphogenic Cell Culture with Osmium Tetroxide, Toluidine Blue O Dye, and Iron Chloride.- Protoplast Isolation, Culture, and Regeneration in Common and Tartary Buckwheat.- Immunohistochemical Detection of the Wall Components on the Example of Shoot Apical Meristem of Fagopyrum esculentum and Fagopyrum tataricum.- Immunodetection of Cell Wall Components in Studies on Cell Wall Rebuilding in Fagopyrum esculentum and Fagopyrum tataricum.- Visualization of Fagopyrum esculentum and Fagopyrum tataricum Chromosomes and Micronuclei.- Embryo Rescue Technique in Buckwheat Fagopyrum esculentum Moench.-Comprehensive Embryological Analyses of Common Buckwheat (Fagopyrym esculentum Moench).- The Phenol-Based Protein Extraction Method for Plant Proteomic Studies.- Two-Dimensional Gel Electrophoresis in Studies of Flower and Leaf Proteome of Common Buckwheat.- Measurement of the Kinetics of Chlorophyll a Fluorescence by an LED-Light Source Fluorimeter, Handy PEA.- Measurements of Leaf Gas-Exchange Parameters Using Portable CIRAS‐3 Infrared Gas Analyzer, with a Parkinson Leaf Chamber (PLC6).- Measurement of the Light Intensity and Spectrum Influence on Plant Growth and Secondary Metabolites of Common Buckwheat.