Xylem (2nd Ed., 2nd ed. 2024) Methods and Protocols Methods in Molecular Biology Series, Vol. 2722
Coordonnateur : Agusti Javier
This second edition provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue. Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem composition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Xylem: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Part I: xylem transport, functional dynamics and modelling
1. Monitoring Xylem Transport in Arabidopsis thaliana Seedlings Using Fluorescent Dyes
Kai Bartusch, Noel Blanco-Touriñán, Antia Rodriguez-Villalon, and Elisabeth Truernit
2. Modeling and analyzing xylem vulnerability to embolism as an epidemic process
Anita Roth-Nebelsick and Wilfried Konrad
3. Modeling Xylem Functionality Aspects
Alex Tavkhelidze, Gerhard Buck-Sorlin, and Winfried Kurth
4. Detecting and quantifying xylem embolism by synchrotron-based x-ray micro-CT
Martina Tomasella, Francesco Petruzzellis, Sara Natale, Giuliana Tromba, and Andrea Nardini
Part II: xylem development and evolution
5. Analysis of xylem cells by nucleus-based transcriptomics and chromatin profiling
Dongbo Shi, Laura Luzzietti, Michael Nodine, and Thomas Greb
6. Quantification of xylem-specific thermospermine-dependent translation of SACL transcripts with dual luciferase reporter system
Anna Solé-Gil, Cristina Úrbez, Alejandro Ferrando, and Miguel A. Blázquez
7. Fossil wood analyses: several examples from five case studies in the area of Central and NW Bohemia, Czech Republic
Jakub Sakala
Part III: xylem diseases
8. Isolation and reproductive structures induction of fungal pathogens associated with xylem and wood necrosis in grapevine
Ana López-Moral and Carlos Agustí-Brisach
9. Determination of de novo suberin-lignin ferulate deposition in xylem tissue upon vascular pathogen attack
Weiqi Zhang, Álvaro Jiménez-Jiménez, Montserrat Capellades, Jorge Rencoret, Anurag Kashyap, and Núria S. Coll
Part IV: xylem composition and imaging
10. Quantification of treacheary elements types in mature hypocotyl of Arabidopsis thaliana
Paula Brunot-Garau, Cristina Urbez, and Francisco Vera-Sirera
11. Histochemical Detection of Peroxidase and Laccase Activities in Populus Secondary XylemMarta-Marina Pérez Alonso, Àngela Carrió-Seguí, and Hannele Tuominen
12. Lignin analysis by HPLC and FTIR: spectra deconvolution and S/G ratio determination
Jorge Reyes Rivera and Teresa Terrazas
13. Inducible pluripotent suspension cell cultures (iPSCs) to study plant cell differentiation
Delphine Ménard, Henrik Serk, Raphael Decou, and Edouard Pesquet
14. Bulk and in situ quantification of coniferaldehyde residues in lignin
Edouard Pesquet, Leonard Blaschek, Junko Takahashi, Masanobu Yamamoto, Antoine Champagne, Nuoendagula, Elena Subitona, Charilaos Dimotakis, Zoltan Bascik, and Shinya Kajita
15. Clearing of vascular tissue in Arabidopsis thaliana for reporter analysis of gene expression
Antonio Serrano-Mislata and Javier BrumósIncludes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 10-2023
Ouvrage de 245 p.
17.8x25.4 cm
Thème de Xylem :
Mots-clés :
systems biology; Microscopy; morphodynamics; vascular development; programmed cell death