Schmidtea Mediterranea, 1st ed. 2023 Methods and Protocols Methods in Molecular Biology Series, Vol. 2680
1. Building Phylogenies from Transcriptomic Data
Lisandra Benítez-Álvarez, Laia Leria, Daniel Dols-Serrate, and Marta Riutort
2. Isolation and Library Preparation of Planarian piRNAs
Iana V. Kim, Tim Demtröder, and Claus-D. Kuhn
3. Genome-Wide Analysis of Planarian piRNAs
Andreas Pittroff, Iana V. Kim, Tim Demtröder, and Claus-D. Kuhn
4. Combining Fluorescent In SituHybridization with Immunofluorescence and Lectin Staining in Planarians
Daniel Font-Martín, Eudald Pascual-Carreras, and Emili Saló
5. Colorimetric Whole-Mount In Situ Hybridization in Planarians
Susanna Fraguas, Mª Dolores Molina, and Francesc Cebrià
6. Single Molecule Fluorescent In SituHybridization (smFISH) on Whole-Mount Planarians
Elke F. Roovers and Kerstin Bartscherer
7. Whole-Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea
Miquel Vila-Farré, Hanh Thi-Kim Vu, and Jochen C. Rink
8. Preparing Planarian Cells for High-Content Fluorescence Microscopy Using RNA In Situ Hybridization and Immunocytochemistry
Markus A. Grohme, Olga Frank, and Jochen C. Rink
9. An RNA/DNA-Based Flow Cytometry Approach for Isolating Slow-Cycling Stem Cells
Nicole Lindsay-Mosher, Alyssa M. Molinaro, and Bret J. Pearson
10. ACME Dissociation-Fixation, Flow Cytometry, and Cell Sorting of Freshwater Planarian Cells
Helena García-Castro, Elena Emili, and Jordi Solana
11. Papain-Based Dissociation of Schmidtea mediterranea Cells
Claudia Ortmeier and Luca Gentile
12. Live Immunostaining and Flow Cytometry of Schmidtea mediterranea Cells
Claudia Ortmeier and Luca Gentile
13. Live Imaging in Planarians: Immobilization and Real-Time Visualization of Reactive Oxygen Species
Vincent Jaenen, Karolien Bijnens, Martijn Heleven, Tom Artois, and Karen Smeets
14. A Planarian Model System to Study Host-Pathogen Interactions
Eli Isael Maciel, Ashley Valle Arevalo, Clarissa J. Nobile, and Néstor J. Oviedo
15. TUNEL Staining in Sections of Paraffin-Enabled Planarians
Maria Rosselló and Teresa Adell
16. Quantitative Analysis of Planarian Pigmentation
Matthew Pittendreigh, Kaleigh Powers, Meenalosini Vimal Cruz, and Jason Pellettieri
17. mRNA Transfection of S. mediterranea Cells for Luminescence Analysis
Uri Weill, Richard Nelson Hall, Leonard Drees, Bo Wang, and Jochen C. Rink
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Date de parution : 07-2023
Ouvrage de 280 p.
17.8x25.4 cm