Functional Analysis of Long Non-Coding RNAs, 1st ed. 2021 Methods and Protocols Methods in Molecular Biology Series, Vol. 2254
Coordonnateur : Cao Haiming
1. Bioinformatics Approaches for Functional Prediction of Long Non-Coding RNAs
Fayaz Seifuddin and Mehdi Pirooznia
2. Visualization of lncRNA and mRNA Structure Models within the Integrative Genomics Viewer
Steven Busan and Kevin M. Weeks
3. RNA Coding Potential Prediction Using Alignment-Free Logistic Regression Model
Ying Li and Liguo Wang
4. Classification of Long Non-Coding RNAs by K-mer Content
Jessime M. Kirk, Daniel Sprague, and J. Mauro Calabrese
5. Genome-Wide Computational Analysis and Validation of Potential Long Non-Coding RNA-Mediated DNA-DNA:RNA Triplexes in the Human Genome
Saakshi Jalali, Amrita Singh, Vinod Scaria, and Souvik Maiti
6. Using INFERNO to Infer the Molecular Mechanisms Underlying Non-Coding Genetic Associations
Alexandre Amlie-Wolf, Pavel P. Kuksa, Chien-Yueh Lee, Elisabeth Mlynarski, Yuk Yee Leung, and Li-San Wang
7. A Bioinformatic Pipeline to Integrate GWAS and eQTL Datasets to Identify Disease Relevant Human Long Non-Coding RNAs
Yi Chen, Ping Li, and Haiming Cao
8. AnnoLnc: A One-Stop Portal to Systematically Annotate Novel Human Long Non-Coding RNAs
De-Chang Yang, Lan Ke, Yang Ding, and Ge Gao
9. Annotation of Full-Length Long Non-Coding RNAs with Capture Long-Read Sequencing (CLS)
Silvia Carbonell Sala, Barbara Uszczyńska-Ratajczak, Julien Lagarde, Rory Johnson, and Roderic Guigo
10. Single-Cell Analysis of Long Non-Coding RNAs (lncRNAs) in Mouse Brain Cells
Boyang Zhang, Wentao Xu, and James Eberwine
11. Detection and Characterization of Ribosome-Associated Long Non-Coding RNAs
Chao Zeng and Michiaki Hamada
12. Analysis of Annotated and Unannotated Long Non-Coding RNAs from Exosome Subtypes Using Next-Generation RNA Sequencing
Wittaya Suwakulsiri, Maoshan Chen, David W. Greening, Rong Xu, and Richard J. Simpson
13. DMS-MaPseq for Genome-Wide or Targeted RNA Structure Probing In Vitro and In Vivo
Phillip Tomezsko, Harish Swaminathan, and Silvi Rouskin
14. Labeling and Purification of Temporally Expressed RNAs during the S-Phase of the Cell Cycle in Living Cells
Matthieu Meryet-Figuiere, Mohamad Moustafa Ali, Santhilal Subhash, and Chandrasekhar Kanduri
15. A Quick Immuno-FISH Protocol for Detecting RNAs, Proteins, and Chromatin Modifications
Akiyo Ogawa and Yuya Ogawa
16. M(R)apping RNA-Protein Interactions
Jasmine Barra, Roberto Vendramin, and Eleonora Leucci
17. In Vivo Administration of Therapeutic Antisense Oligonucleotides
Luisa Statello, Mohamad Moustafa Ali, and Chandrasekhar Kanduri
18. CRISPR-Mediated Mutagenesis of Long Non-Coding RNAs
Tomohiro Yamazaki and Tetsuro Hirose
19. In Vivo CRISPR/Cas9-Based Targeted Disruption and Knock-In of a Long Non-Coding RNA
Xi Cheng, Samuel T. Peters, Shondra M. Pruett-Miller, Thomas Saunders, and Bina Joe
20. Genome-Scale Perturbation of Long Non-Coding RNA Expression Using CRISPR Interference
S. John Liu, Max A. Horlbeck, Jonathan S. Weissman, and Daniel A. Lim
21. In Vivo Functional Analysis of Non-Conserved Human lncRNAs Using a Humanized Mouse Model
Yonghe Ma, Cheng-fei Jiang, Ping Li, and Haiming Cao
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Date de parution : 12-2021
Ouvrage de 351 p.
17.8x25.4 cm
Date de parution : 12-2020
Ouvrage de 351 p.
17.8x25.4 cm