Coronaviruses (2nd Ed., 2nd ed. 2020) Methods and Protocols Methods in Molecular Biology Series, Vol. 2203
Coordonnateurs : Maier Helena J., Bickerton Erica
1. Coronaviruses: An Updated Overview of Their Replication and Pathogenesis
Yuhang Wang, Matthew Grunewald, and Stanley Perlman
Part I: Coronavirus Detection, Discovery, and Evolution
2. Discovery of Novel Coronaviruses in Rodents
Theocharis Tsoleridis and Jonathan K. Ball
3. Detection and Discovery of Coronaviruses in Wild Bird Populations
Chantal J. Snoeck and Siamak Zohari
4. Competitive ELISA for the Detection of Serum Antibodies Specific for Middle East Respiratory Syndrome-Coronavirus (MERS-CoV)
Shuetsu Fukushi
5. Whole Genome Sequencing Protocols for IBV and Other Coronaviruses Using High-Throughput Sequencing
Graham L. Freimanis and Michael S. Oade
Part II: Propagation and Titration of Coronaviruses
6. Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Study
Hui Hu, Kwonil Jung, Scott P. Kenney, and Linda J. Saif
7. The Preparation of Chicken Kidney Cell Cultures for Virus Propagation
Elena Lokhman, Srijana Rai, and Billy Matthews
8. The Preparation of Chicken Tracheal Organ Cultures and Their Application for Ciliostasis Test, Growth Kinetics Studies, and Virus Propagation
Giulia Dowgier and Erica Bickerton
9. Isolation and Propagation of Coronaviruses in Embryonated Eggs
James S. Guy
10. Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures
Mitra Gultom, Laura Laloli, and Ronald Dijkman
11. Quantification of Coronaviruses by Titration In Vitro and Ex Vivo
Giulia Dowgier and Helena J. Maier
Part III: Manipulating the Genomes of Coronaviruses
12. Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses
Sarah Keep, Paul Britton, and Erica Bickerton
13. In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline
Tran Thi Nhu Thao, Fabien Labroussaa, Nadine Ebert, Joerg Jores, and Volker Thiel
Part IV: Studying Virus-Host Interactions
14. Proximity Labeling for Identification of Coronavirus-Host Protein Interactions
Philip V’kovski, Silvio Steiner, and Volker Thiel
15. Using Yeast to Identify Coronavirus-Host Protein Interactions
Stuart Weston and Matthew Frieman
16. Determining How Coronaviruses Overcome the Interferon and Innate Immune Response
Christopher M. Coleman
17. Ribopuromycylation in Coronavirus-Infected Cells
Matthew J. Brownsword, Helena J. Maier, and Nicolas Locker
Part V: Imaging Coronavirus Infections
18. Visualizing Coronavirus Entry into Cells
Aleksandra Milewska, Katarzyna Owczarek, Artur Szczepanski, and Krzysztof Pyrc
19. Preparation of Cultured Cells Using High Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope
Nicole Doyle and Philippa C. Hawes
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Date de parution : 08-2021
Ouvrage de 278 p.
17.8x25.4 cm
Date de parution : 08-2020
Ouvrage de 278 p.
17.8x25.4 cm