Biophysics of Membrane Proteins, 1st ed. 2020 Methods and Protocols Methods in Molecular Biology Series, Vol. 2168
Coordonnateurs : Postis Vincent L. G., Goldman Adrian
This volume provides recent advances in the field of biophysics of membrane proteins. Chapters are divided into several parts: detailing biochemistry and functional analysis, experimental and theoretical structural determinations, membrane protein dynamics, and conformation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Biophysics of Membrane Proteins: Methods and Protocols aims to provide comprehensive protocols with notes to help further the understanding of key membrane protein structure and function for students, academics, and industrial researchers.
Part I: Biochemistry and Functional Analysis
1. Membrane Protein Production and Purification from Escherichia coli and Sf9 Insect Cells
Yixin Liu, Ana Pavić, Joshua T. Farley, Carine de Marcos Lousa, Adrian Goldman, and Vincent L. G. Postis
2. Quantifying the Interaction of Phosphite with ABC Transporters: MicroScale Thermophoresis and a Novel His-tag Labeling Approach
Tanja Bartoschik, Amit Gupta, Beate Kern, Andrew Hitchcock, Nathan B.P. Adams, and Nuska Tschammer
3. Rationale for the Quantitative Reconstitution of Membrane Proteins into Proteoliposomes
Dhenesh Puvanendran, Hager Souabni, Dimitri Salvador, Olivier Lambert, Quentin Cece, and Martin Picard
4. Functional Characterization of SLC transporters using Solid-Supported-Membranes
Andre Bazzone and Maria Barthmes
5. Thermostability Assays Provide a Generic and Versatile Tool for Studying the Functional and Structural Properties of Membrane Proteins in Detergents
Steven P.D. Harborne, Martin S. King, and Edmund R.S. Kunji
6. Direct monitoring of GPCR reconstitution and ligand binding activity by Plasmon Waveguide Resonance
Isabel D. Alves
Part II: Experimental and Theoretical Structural Determination
7. Examining Membrane Proteins by Neutron Scattering
Christine Ebel, Cécile Breyton, and Anne Martel
8. Solution X-ray Scattering for Membrane Protein
Maciej Baranowski and Javier Pérez
9. Interpreting SAXS/WAXS Data with Explicit-Solvent Simulations: A Practical Guide
Markus Rainer Hermann and Jochen Sebastian Hub
10. Determining the Free Energies of Outer Membrane Proteins in Lipid Bilayers
Gerard H M Huysmans, Dagan C Marx, Sheena E Radford,and Karen G Fleming
11. Interrogating Membrane Protein Structure and Lipid Interactions by Native Mass Spectrometry
Dietmar Hammerschmid, Jeroen F. van Dyck, Frank Sobott, and Antonio N. Calabrese
12. Determination of the Molecular Mass of Membrane Proteins Using Size Exclusion Chromatography with Multi-angle Laser Light Scattering (SEC-MALLS)
Maren Thomsen
Part III: Membrane Protein Dynamics and Conformations
13. Dynamics of Membrane Proteins Monitored by Single-molecule Fluorescence across Multiple Time Scales
Tomas Fessl, Matthew A Watson, Daniel Watkins, Marek Scholz, Joel Crossley, Tara Sabir, Sheena E. Radford, Ian Collinson, and RomanTuma
14. Short-Range Distances Measurement by Transition Metal Ion FRET
Jonas S. Mortensen and Claus J. Loland
15. PELDOR/DEER – An Electron Paramagnetic Resonance Method to Study Membrane Proteins in Lipid Bilayers
Martin F. Peter, Kiran Bountra, Konstantinos Beis, and Gregor Hagelueken
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 02-2022
Ouvrage de 336 p.
17.8x25.4 cm
Date de parution : 02-2021
Ouvrage de 336 p.
17.8x25.4 cm
Thème de Biophysics of Membrane Proteins :
Mots-clés :
SEC-MALLS; cryoEM; Free Electron Lasers; MD simulations; neutron scattering