Axon Degeneration, 1st ed. 2020 Methods and Protocols Methods in Molecular Biology Series, Vol. 2143
Coordonnateur : Babetto Elisabetta
Part I: Introduction
1. Axon Degeneration: Which Method to Choose?
Michael P. Coleman
Part II: In Vitro Assays
2. Axon Degeneration Assays in Superior Cervical Ganglion Explant Cultures
Andrea Loreto and Jonathan Gilley
3. Microinjection of Superior Cervical Ganglion Neurons for Studying Axon Degeneration
Jonathan Gilley and Andrea Loreto
4. Assessing Axonal Degeneration in Embryonic Dorsal Root Ganglion Neurons In Vitro
Jung Eun Shin and Yongcheol Cho
5. Viral Transduction of DRG Neurons
Yo Sasaki
6. Planning and Analysis of Axon Degeneration Screening Experiments
Lyndah Lovell, Jack Bramley, and William Buchser
7. A Microfluidic Culture Platform to Assess Axon Degeneration
Yu Yong, Christopher Hughes, and Christopher Deppmann
8. A Schwann Cell-Neuron Co-Culture System to Study Neuron-Glia Interaction during Axon Degeneration
Elisabetta Babetto
9. Establishing Myelinating Co-Cultures Using Human iPSC-Derived Sensory Neurons to Investigate Axonal Degeneration and Demyelination
Alex Clark
Part III: Ex Vivo Assays
10. Preparation of Organotypic Hippocampal Slice Cultures for the Study of CNS Disease and Damage
Claire S. Durrant
11. Organotypic Culture Assay for Neuromuscular Synaptic Degeneration and Function
Kosala N. Dissanayake, Robert Chang-Chih Chou, Rosalind Brown, and Richard R. Ribchester
12. Intracellular Recordings of Postsynaptic Voltage Responses at the Drosophila Neuromuscular Junction
Vera Valakh and Johanna G. Flyer-Adams
13. Ex Vivo Studies of Optic Nerve Axon Electrophysiology
Chinthasagar Bastian, Sylvain Brunet, and Selva Baltan
14. Ex Vivo Analysis of Axonal Degeneration Using Sciatic and Optic Nerve Preparations
Rodrigo López-Leal and Felipe A. Court
15. Measuring Bioenergetic Signatures of Peripheral Nerve Segments by Extracellular Flux Analysis
Bogdan Beirowski
Part IV: In Vivo Assays
16. Detection of Neutrophils in the Sciatic Nerve Following Peripheral Nerve Injury
Jon P. Niemi, Jane A. Lindborg, and Richard E. Zigmond
17. Whole-Tissue Immunolabeling and 3D Fluorescence Imaging to Visualize Axon Degeneration in the Intact, Unsectioned Mouse Tissues
Ying Cao and Jing Yang
18. Transmission Electron Microscopy and Morphometry of the CNS White Matter
Julia M. Edgar, Rebecca Sherrard Smith, and Ian D. Duncan
19. In Vivo Calcium Imaging During Axon Degeneration in Zebrafish
Mauricio E. Vargas
20. In Vivo Imaging of Anterograde and Retrograde Axonal Transport in Rodent Peripheral Nerves
James N. Sleigh, Andrew P. Tosolini, and Giampietro Schiavo
21. In Vivo Visualization of Moving Synaptic Cargo Complexes within Drosophila Larval Segmental Axons
Rupkatha Banerjee, Joseph A. White II, and Shermali Gunawardena
22. A Chemotherapy-Induced Peripheral Neuropathy Model in Drosophila melanogaster
Martha R.C. Bhattacharya
23. Models of Axon Degeneration inDrosophilaLarvae
E.J. Brace and Aaron DiAntonio
24. In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster
Mary A. Logan and Sean D. Speese
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Date de parution : 06-2020
Ouvrage de 340 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 126,59 €
Ajouter au panierDate de parution : 06-2020
Ouvrage de 340 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 179,34 €
Ajouter au panier