In Vitro Differentiation of T-Cells, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 2048

1. Flow Cytometry Analysis to Identify Human CD8⁺ T-Cells

            Jacqueline Flynn and Paul Gorry

2. Flow Cytometry Analysis to Identify Human CD4⁺ T-Cell Subsets

            Jacqueline Flynn and Paul Gorry

3. Gene Modification and Immunological Analyses for the Development of Immunotherapy Utilizing T-Cells Redirected with Antigen-Specific Receptors

            Toshiki Ochi, Masaki Maruta, and Naoto Hirano

4. In Vitro Conversion of Activated T-Cells into Stem Cell Memory-Like T-Cells

            Taisuke Kondo, Yuuki Imura, Makoto Ando, Shunsuke Chikuma, and Akihiko Yoshimura

5. Human iPSC Generation from Antigen-Specific T-Cells

            Toshinobu Nishimura, Yukiko Murmann, and Hiromitsu Nakauchi

6. In Vitro Differentiation of T-Cells: From Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

            Shoichi Iriguchi and Shin Kaneko

7. Redifferentiation of Adaptive Naïve-Like CTL from T-Cell-Derived iPSC

            Yohei Kawai and Shin Kaneko

8. In Vitro Differentiation of T-Cell: From Human iPS Cells in Feeder Free Condition

            Yutaka Yasui, Yasumichi Hitoshi, and Shin Kaneko

9. Differentiating CD8ab T-Cells from TCR Transduced iPSCs for Cancer Immunotherapy

            Atsutaka Minagawa and Shin Kaneko

10. In Vitro Differentiation of T-Cell: From CAR-Modified T-iPSC

            Tatsuki Ueda and Shin Kaneko

11. In Vitro Differentiation of T-Cells: From Non-Human Primate Induced Pluripotent Stem Cells

            Shoichi Iriguchi and Shin Kaneko

12. An Improved Method to Produce Clinical Scale Natural Killer Cells from Human Pluripotent Stem Cells

            Huang Zhu and Dan S. Kaufman

13. In Vitro Detection of Cellular Adjuvant Properties of Human Invariant Natural Killer T-Cells

            Rong Zhang, Shuichi Kitayama, Tianyi Liu, Norihiro Ueda, Yumi Tokumitsu, Hiroaki Mashima, Hideki Ohdan, Shin Kaneko, and Yasushi Uemura

14. In Vitro Differentiation of T-Cells from Murine Pluripotent Stem Cells

            Xiaoniao Chen, Fengyang Lei, Liqiang Wang, Xiaofang Xiong, and Jianxun Song

15. Clonogenic Culture of Mouse Thymic Epithelial Cells

            Miho Sekai, Jianwei Wang, and Yoko Hamazaki

16. Single-Cell Transcriptome Analysis of T-Cells

            Willem Van Der Byl, Simone Rizzetto, Jerome Samir, Curtis Cai, Auda A. Eltahla, and Fabio Luciani

17. Structural Modeling of Lymphocyte Receptors and Their Antigens

            Songling Li, Jan Wilamowski, Shunsuke Teraguchi, Floris J. van Eerden, John Rozewicki, Ana Davila, Zichang Xu, Kazutaka Katoh, and Daron M. Standley

18. Assessing T Lymphocyte Aging Using Telomere Length and Telomerase Activity Measurements in Low Cell Numbers

            Tinhinane Fali, Camille K'Ros, Victor Appay, and Delphine Sauce

19. Generation of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells

            Hideyuki Oguro

20. Using the Inducible Caspase9 Suicide-Safeguard System with iPSC and Bioluminescent Tracking

            Jonathan Villanueva, Toshinobu Nishimura, and Hiromitsu Nakauchi

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts