Chiral Recognition and Separation Mechanisms Chromatographic Science Series

Chirality plays a major role in biological processes and the enantiomers of a bioactive molecule often possess different biological effects. Accurate assessment is critical as isomeric impurities may have unwanted toxicological, pharmacological, or other effects. Separation techniques remain the method of choice for this area of drug research but there is a great deal of trial and error in choosing a particular column for a chiral separation. This book gives a rationale for choosing a stationary phase that is based on the relationship that exists between the analytes and the chiral stationary phases. The text provides examples to underline the separation mechanism.

Introduction. Symmetry. Separation of Enantiomers through the Formation of Diastereomers. Non-chromatographic Methods for the Determination of Enantiomeric Excess. Mechanism of Chromatographic Chiral Discrimination & Thermodynamics of Chromatographic Chiral Discrimination. Chiral Separation with Taylor Made Polymers. Chiral Separation through Ligand Exchange. Chiral Separation through H-bonds. Chiral Separation through Charge Transfer. Chiral Separation through Inclusion Compounds. Protein Phases and Antibiotic Phases. Polysaccharide Phases. Separation of Enantiomers with Chiral Mobile Phases. Strategies for Method Development for Chiral Separation. Conclusion/Summary and Future Possibilities.

Nelu Grinberg is with Boehringer Ingelheim Pharmaceuticals, in Connecticut, USA.