DNA Topoisomerases, Softcover reprint of the original 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1703
Coordonnateur : Drolet Marc
This volume looks at the evolution of the topo field over the last year and discusses how DNA topoisomerases are directly and indirectly involved in numerous cellular processes. The chapters in this book cover various topics, such as type 1 topos with an evolutionary perspective; antimicrobial agents targeting topos; experimental protocols related to DNA topology, including a basic technique to measure DNA supercoiling in bacteria; protocols for in vitro studies, including assays to detect RNA topo activity; topo inhibitors; and a study of plasmid-mediated quinolone resistance in bacteria. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, DNA Topoisomerases: Methods and Protoc
Preface…
Table of Contents…
Contributing Authors…
1- Type IA DNA Topoisomerases: A Universal Core and Multiple Activities
Florence Garnier, Hélène Debat, and Marc Nadal
2- Topoisomerase I and Genome Stability: The Good and The Bad
Jang-Eun Cho and Sue Jinks-Robertson
3- DNA Topoisomerases as Targets for Antibacterial Agents
Hiroshi Hiasa
4- DNA Supercoiling Measurement in Bacteria
Yingting Liu, Zhi-Chun Hua, and Fenfei Leng
5- DNA Catenation Reveals the Dynamics of DNA Topology during Replication
Alicia Castán, Pablo Hernández, Dora B. Krimer, and Jorge B. Schvartzman
6- Mapping E. coli Topoisomerase IV Binding and Activity Sites
Hafez El Sayyed and Olivier Espéli
7- The Use of Psoralen Photobinding to Study Transcription-Induced Supercoiling
Fedor Kouzine, Laura Baranello, and David Levens
8- Immunoprecipitation of RNA:DNA Hybrids from Budding Yeast
Aziz El Hage and David Tollervey
9- Detect
Makisha Martel, Aurélien Balleydier, Julien Brochu, and Marc Drolet
10- Single-Molecule Magnetic Tweezer Analysis of Topoisomerases
Kathryn H. Gunn, John F. Marko, and Alfonso Mondragón
11- Synthesis of Hemicatenanes for the Study of Type IA Topoisomerases
Shun-Hsiao Lee, Tao-shih Hsieh, and Grace Ee-Lu Siaw
12- An Assay for Detecting RNA Topoisomerase Activity
Muzammil Ahmad, Dongyi Xu, and Weidong Wang
13- Studying TDP1 Function in DNA Repair
Shih-Chieh Chiang, Kirsty Liversidge, and Sherif F. El-Khamisy
14- Topoisomerase II Chromatin Immunoprecipitation
Kayleigh A. Smith, Ian G. Cowell, and Caroline A. Austin
15- Analyzing Mitotic Chromosome Structural Defects after Topoisomerase II Inhibition or Mutation
Juan F. Giménez-Abián, Andrew B. Lane, and Duncan J. Clarke
16- Monitoring the DNA Topoisomerase II Checkpoint in Saccharomyces cerevisiae
Katherine Furniss, Amit C. J. Vas, Andrew Lane, and Duncan J. Clarke
17- Studying Topoisomerase 1-Mediated Damage at Genomic Ribonucleotides
Jessica S. Williams and Thomas A. Kunkel
18- A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons
Thirunavukkarasu Annamalai, Bokun Cheng, and Yuk-Ching Tse-Dinh
19- Fluoroquinolone-Gyrase-DNA Cleaved Complexes
Gan Luan and Karl Drlica
20- Detection of Topoisomerase Covalent Complexes in Eukaryotic Cells
Jay Anand, Yilun Sun, Yang Zhao, Karin C. Nitiss, and John L. Nitiss
21- Visualization and Quantification of Topoisomerase-DNA Covalent Complexes Using the Trapped in Agarose Immunostaining (TARDIS) Assay
Ian G. Cowell and Caroline A. Austin
22- Study of Plasmid-Mediated Quinolone Resistance in Bacteria
George A. Jacoby
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Ouvrage de 328 p.
17.8x25.4 cm
Ouvrage de 328 p.
17.8x25.4 cm
Mots-clés :
genome stability; supercoiled DNA; chromosome segregation; Cre/loxP; Purification; topoisomerases
