Mathematical Modeling and Scale-up of Liquid Chromatography

Liquid chromatography has proved to be one of the most important tools for separations. Rapid development in biotechnology has increased the demand for chromatography in analytical, preparative and large scale applications. The understanding of the dynamics of chromatography is imperative for the scale-up. This book is a systematic treatment of the general rate models for various forms of liquid chromatography including adsorption, size exclusion, affinity, reversed phase, hydrophobic interaction, and radial flow chromatography. Thermodynamic and mass transfer effects in liquid chromatography are discussed. Applications of computer programs for the rate models are described and the procedures for the scale-up of preparative- and large-scale liquid chromatography using the general rate models are given.

1 Introduction.- 2 Literature Review.- 2.1 Theories for Nonlinear Multicomponent Liquid Chromatography.- 2.2 Scale-Up of Liquid Chromatography.- 3 A General Multicomponent Rate Model for Column Liquid Chromatography.- 3.1 Model Assumptions.- 3.2 Model Formulation.- 3.3 Finite Element Formulation for the Bulk-Fluid Phase Governing Equation.- 3.4 Orthogonal Collocation Formulation of the Particle Phase Governing Equation.- 3.5 Solution to the ODE System.- 3.6 Fortran 77 Code for the General Multicomponent Rate Model.- 3.7 CPU Time for the Simulation.- 3.8 Extension of the General Multicomponent Rate Model.- 3.9 The Question of Choosing Column Boundary Conditions.- 4 Mass Transfer Effects.- 4.1 Effects of Parameters PeLi, Bii and ?i.- 4.2 Effect of Flow Rate.- 4.3 Effect of Mass Transfer in a Case with Unfavorable Isotherm.- 5 Interference Effects in Multicomponent Chromatography.- 5.1 Introduction.- 5.2 Computer Simulation and Discussion.- 5.3 Summary.- 6 System Peaks In Multicomponent Elution.- 6.1 Introduction.- 6.2 Boundary Conditions for the General Rate Model.- 6.3 Results and Discussion.- 6.4 Concluding Remarks.- 7 Multicomponent Adsorption with Uneven Saturation Capacities.- 7.1 Introduction.- 7.2 Kinetic and Isotherm Models.- 7.3 Isotherm Crossover.- 7.4 Summary.- 8 Modeling of Affinity Chromatography.- 8.1 Introduction.- 8.2 Effect of Reaction Kinetics.- 8.3 Effect of Size Exclusion.- 8.4 Interaction Between Soluble Ligand and Macromolecule.- 8.5 Modeling of the Three Stages in Affinity Chromatography.- 8.6 How to Use the Fortran 77 Code AFFINITY.F.- 8.7 Summary.- 9 Modeling of Multicomponent Gradient Elution.- 9.1 Introduction.- 9.2 General Rate Model for Multicomponent Gradient Elution.- 9.3 Numerical Solution.- 9.4 How to Use the Fortran 77 Code GRADIENT.F.-9.5 Summary.- 10 Multicomponent Radial Flow Chromatography.- 10.1 Introduction.- 10.2 General Multicomponent Rate Model for RFC.- 10.3 Numerical Solution.- 10.4 How to Use the Fortran 77 Code RATERFC.F.- 10.5 Extensions of the General Multicomponent Rate Model for RFC.- 10.6 Summary.- 11 Scale-Up of Liquid Chromatography using General Rate Models.- 11.1 Isotherms.- 11.2 Mass Transfer Parameters.- 11.3 Evaluation of Pei, ?i, and Bii,.- 11.4 General Procedure for Scale-Up.- 12 References.- 13 Subject Index.