Rapid Cycle Real-Time PCR — Methods and Applications, Softcover reprint of the original 1st ed. 2004 Quantification
Coordonnateurs : Wittwer Carl, Hahn Meinhard, Kaul Karen
Rapid cycle real-time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybridization probes (HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!
Date de parution : 10-2012
Ouvrage de 223 p.
19.3x24.2 cm
Date de parution : 06-2004
Ouvrage de 200 p.
Thème de Rapid Cycle Real-Time PCR — Methods and Applications :
Mots-clés :
DNA; LightCycler; PCR; Quantification; RNA; gene expression; transcription