T-Cell Trafficking (2^nd Ed., 2nd ed. 2017) Methods and Protocols Methods in Molecular Biology Series, Vol. 1591

This second edition provides updated and new chapters on T-Cell trafficking. In addition to detailed experimental procedures, the interested reader will find informative introductory chapters on the relevance of T-Cell trafficking in thymic population and maturation, traffic through secondary lymphoid organs during ?physiological? resolving inflammation and during immune responses, as well as T-Cell trafficking in chronic inflammatory diseases. Importantly, chapters cover methods from in silico modeling of cellular interactions, in vitro adhesion assays, through ex vivo functional assays to integrated intravital modeling of T-Cell trafficking through organs. Written in the highly successful Methods in Molecular Biology series format, each methods chapter includes a short introduction to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, T-Cell Trafficking: Methods and Protocols, Second Edition aims to be an essential point of reference for those new to the field of T-Cell trafficking, or to those looking to expand their technical capabilities.

1. Introduction to Homeostatic Migration

Mark C. Coles

2. Analysis of Thymocyte Migration, Cellular Interactions, and Activation by Multi-Photon Fluorescence Microscopy of Live Thymic Slices

Jessica N. Lancaster and Lauren I. R. Ehrlich

3. Visualising and Tracking T Cell Motility in vivo

Robert A Benson, James M Brewer, and Paul Garside

4. Graph Theory-based Analysis of the Lymph Node Fibroblastic Reticular Cell Network

Mario Novkovic, Lucas Onder, Gennady Bocharov, and Burkhard Ludewig

5. Visualizing Endogenous Effector T Cell Egress from the Lymph Nodes

Manisha Menon, Alexandre P Benechet, and Kamal M Khanna

6. Introduction: T cell Trafficking in Inflammation and Immunity

Myriam Chimen, Bonita H. R. Apta, and Helen M. McGettrick

7. Leukocyte Adhesion under Haemodynamic Flow Conditions

Charlotte Lawson, Marlene Rose, and Sabine Wolf

Bonita H. R. Apta, Myriam Chimen, and Helen M. McGettrick

9. Mesenchymal Stromal Cells as Active Regulators of Lymphocyte Recruitment to Blood Vascular Endothelial Cells

10. Monitoring RhoGTPase Activity in Leukocytes using Classic “pull-down” Assays

Marouan Zarrouk, David Killock, Izajur Rahman, Jessica Davies, and Aleksandar Ivetić

11. Utilising Lentiviral Gene Transfer in Primary Endothelial Cells to Assess Lymphocyte-Endothelial Interactions

Jasmeet S. Reyat, Michael G. Tomlinson, and Peter J. Noy

12. Introduction to Lymphocyte Trafficking in Disease

Patricia F. Lalor and Elizabeth Hepburn

13. Using Ex-Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking

Ben Wiggins, Zania Stamataki, and Patricia F. Lalor

14. In vitro and Ex Vivo Models to Study T Cell Migration through the Human Liver Parenchyma

Benjamin G. Wiggins, Konstantinos Aliazis, and Scott P. Davies

15. Monitoring Migration of Activated T Cells to Antigen-rich Non-lymphoid Tissue

Eleanor Jayne Ward, Hongmei Fu, and Federica Marelli Berg

16. Tissue Digestion for Stromal Cell and Leukocyte Isolation

Saba Nayar, Joana Campos, Nathalie Steinthal, and Francesca Barone

17. T-cell Response in the Lung Following Influenza Virus Infection

Robert A. Benson, Jennifer C. Lawton, and Megan K.L. MacLeod

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras