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Phage Display, 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1701

Langue : Anglais

Coordonnateurs : Hust Michael, Lim Theam Soon

Couverture de l’ouvrage Phage Display
This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field
of antibody phage display. This volume will also aid in stirring interest and ideas among researchers in this ever-expanding subject.

Table of Contents…
Contributing Authors…

Part I Construction of Antibody Phage Display Libraries

1. Construction of Human Immune and Naïve scFv Libraries
Jonas Kügler, Florian Tomszak, André Frenzel, and Michael Hust

2. Construction of Naïve and Immune Human Fab Phage Display Library
Noorsharmimi Omar and Theam Soon Lim

3. Construction of Synthetic Antibody Phage Display Libraries
Johan Nilvebrant and Sachdev S. Sidhu

4. Modular Construction of Large Non-Immune Human Antibody Phage Display Libraries from Variable Heavy and Light Chain Gene Cassettes
Nam-Kyung Lee, Scott Bidlingmaier, Yang Su, and Bin Liu

5. Construction of Macaque Immune Libraries
Arnaud Avril, Sebastian Miethe, Michael Hust, and Thibaut Pelat

6. Construction o
f Bovine Immunoglobulin Libraries in the single chain Fragment variable (scFv) Format
Ulrike S. Diesterbeck

7. Construction of Rabbit Immune Antibody Libraries
Thi Thu Ha Nguyen, Jong Seo Lee, and Hyunbo Shim

8. Generation of Semi-Synthetic Shark IgNAR Single Domain Antibody Libraries
Julius Grzeschik, Doreen Könning, Simon Krah, Christian Schröter, Martin Empting, Harald Kolmar, and Stefan Zielonka

9. Construction of High-Quality Camel Immune Antibody Libraries
Ema Romão, Vianney Poignavent, Cécile Vincke, Christophe Ritzenthaler, Serge Muyldermans, and Baptiste Monsion

10. Construction of Chicken Antibody Libraries
Jeanni Fehrsen, Susan Wemmer, and Wouter van Wyngaardt

11. Construction and Selection of Affilin Phage Display Libraries
Florian Settele, Madlen Zwarg, Sebastian Fiedler, Daniel Koscheinz, and Eva

12. Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies
Déborah Caucheteur, Gautier Robin, Vincent Parez, and Pierre Martineau

13. Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach
Zhihao Wu, Brian H. Santich, Hong Liu, Cheng Liu, and Nai-Kong V. Cheung

Part II Selection Strategies for Antibodies

14. Parallelized Antibody Selection in Microtiter Plates
Giulio Russo, Doris Meier, Saskia Helmsing, Esther Wenzel, Fabian Oberle, André Frenzel, and Michael Hust

15. Mass Spectrometry Immuno Assay (MSIA ™) Streptavidin Disposable Automation Research Tips (D.A.R.T’s®) Antibody Phage Display Biopanning
Chai Fung Chin, Yee Siew Choong, and Theam Son Lim

16. Magnetic Nanoparticle-Based Semi-Automated Panning for
High-Throughput Antibody Selection
Angela Chiew Wen Ch’ng, Nurul Hamizah binti Hamidon, Zoltán Konthur, and Thean Soon Lim

17. Phage Display and Selections on Cells
Wieland Fahr and André Frenzel

18. Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens
Yang Su, Scott Bidlingmaier, Nam-Kyung Lee, and Bin Liu

19. Antibody Isolation Form a Human Synthetic Combinational and Other Libraries of Single-Chain Antibodies
Almog Bitton, Limor Nahary, and Itai Benhar

20. Screening Phage Display Antibody Libraries using Protein arrays
Ricardo Jara-Acevedo, Paula Díez, María González-González, Rosa María Dégano, Nieves Ibarrola, Rafael Góngora, Alberto Orfao, and Manuel Fuentes

21. Antibody
Selection on FFPE Tissue Slides
Andre ten Haaf, Stefan Gattenlöhner, and Mehmet Kemal Tur

22. Antibody Affinity and Stability Maturation by Error Prone PCR
Tobias Unkauf, Michael Hust, and André Frenzel

Part III Complementary Approaches for Antibody Phage Display Selections

23. Upgrading Affinity Screening Experiments by Analysis of Next Generation Sequencing Data 
Christian Grohmann and Michael Blank

24. Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Library Construction
Kevin A. Henry

25. High-Throughput IgG Reformatting and Expressions
Chao-Guang Chen, Georgina Sansome. Michael J. Wilson, and Con Panousis

26. Monitoring Phage Biopanning by Next-Generation Sequencing
Anna Vaisman-Mentesh and Yariv Wine
Part IV Phage Display for Epitope Mapping and Identification of Biomarkers

27. ORFeome Phage Display
Jonas Zantow, Gustavo Marçal Schmidt Garcia Moreira, Stefan Dübel, and Michael Hust

28. Epitope Mapping by Phage Display
Gustavo Marçal Schmidt Garcia Moreira, Viola Fühner, Michael Hust

29. Metasecretome Phage Display
Milica Ciric, Filomena Ng, Jasna Rakonjac, and Dragana Gagic

30. Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on Phages
Gertrudis Rojas and Tania Carmenate

Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts

Date de parution :

Ouvrage de 562 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

Prix indicatif 189,89 €

Ajouter au panier

Date de parution :

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

Prix indicatif 189,89 €

Ajouter au panier
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