Pancreatic Cancer (3^rd Ed., 3rd ed. 2019) Methods and Protocols Methods in Molecular Biology Series, Vol. 1882

This third edition volume expands on the previous editions with discussions on the latest developments in 2D- and 3D-organoid cultures that allow for the execution of precision medicine, and in vitro studies of tumor cell growth and invasion. The chapters in this book explore protocols for evaluating macropinocytosis, stress granules, autophagy, metabolic alterations, and reactive oxygen species, which have proven to be possible therapeutic opportunities. The protocols described in this book look at both human and animal models, ranging from in vitro and in vivo, and will aid in future research of pancreatic cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Pancreatic Cancer: Methods and Protocols, Third Edition is a valuable resource for scientists and researchers who are interested in learning more about ways of improving patient care for pancreatic cancer.

Preface…

Table of Contents…

Contributing Authors…

1. Subtyping of IPMN

Toru Furukawa

2. Clinical Management and Surgical Decision-Making of IPMN of the Pancreas

Masao Tanaka

3. Develop Nomogram to Predict Malignancy of Intraductal Papillary Mucinous Neoplasm

Yongkang Kim and Taesung Park

4. Generation of Induced Pluripotent Stem Cell-Like Lines from Human Pancreatic Ductal Adenocarcinoma

Jungsun Kim and Kenneth S. Zaret

5. Lineage Tracing of Primary Human Pancreatic Acinar and Ductal Cells for Studying Acinar to Ductal Metaplasia

Jun Liu and Pei Wang

6. Spheroid Culture of Human Pancreatic Ductal Cells to Reconstitute Development of Pancreatic Intraepithelial Neoplasia

James J. Lee  and Seung K. Kim

7. Pancreatic Microtumors: A Novel D Ex Vivo Testing Platform

M.L. Goodwin, S.K. Urs, and D.M. Simeone

8. 3D Models of Pancreatic Ductal Adenocarcinoma via Tissue Engineering

Claudio Ricci and Serena Danti

 

9. Generation and Culture of Human Pancreatic Ductal Adenocarcinoma Organoids from Resected Tumor Specimens

Lindsey A. Baker, Hervé Tiriac, and David A. Tuveson

10. Generation and Culture of Tumor and Metastatic Organoids from Murine Models of Pancreatic Ductal Adenocarcinoma

Lindsey A. Baker and David A. Tuveson

11. An In Vitro Three-Dimensional Organotypic Model to Analyze Peripancreatic Fat Invasion in Pancreatic Cancer: A Culture System Based on Collagen Gel Embedding

Takashi Okumura, Kenoki Ohuchida, and Masafumi Nakamura

12. Osteoclasts Differentiation Assay

Jingxuan Yang, Xiaohong Bi, and Min Li

13. Primary Cultures for Pancreatic Stellate Cells (PSCs)

Lei Tian, Zipeng Lu, and Yi Miao

14. Cytokine Profiling and Orthotopic Xenografing of Pancreatic Stellate Cells

15. Quantitative Method to Track Proteolytic Invasion in 3D Collagen

Kazumi Ebine, Christina R. Chow, and Hidayatullah G. Munshi

16. Detection and Quantification of Macropinosomes in Pancreatic Tumors

17. Evaluating Stress Granules in Pancreatic Cancer In Vitro and In Vivo

Edward Sim, Elena Irollo, and Elda Grabocka

18. Methods for Monitoring Macroautophagy in Pancreatic Cancer Cells

Chiara Vidoni, Alessandra Ferraresi, Christian Seca, Eleonora Secomandi, and Ciro Isidoro

19. Measurement of Reactive Oxygen Species by Fluorescent Probes in Pancreatic Cancer Cells

Yongde Luo, Dan Wang , James L. Abbruzzese, and Weiqin Lu

20. Evaluating the Metabolic Alterations in Pancreatic Cancer

21. Isolation of Extracellular Vesicles for Cancer Diagnosis and Functional Studies

Alex W. Brenner, Gloria H. Su, and Fatemeh Momen-Heravi

22. Evaluation of Post-Transcriptional Gene Regulation in Pancreatic Cancer Cells: Studying RNA Binding Proteins and their mRNA Targets

Aditi Jain, Samantha Brown, Henry L. Thomsett, Eric Londin, and Jonathan R. Brody 

23. Laser Capture Microdissection on Frozen Sections for Extraction of High-Quality Nucleic Acids

H. Carlo Maurer and Kenneth P. Olive

24. Statistical Methods for Identifying Biomarkers from miRNA Profiles of  Cancers

Junghyun Namkung 

25. Induction of Pancreatic Inflammation Accelerates Pancreatic Tumorigenesis in Mice

Lu Zhuang, Xianbao Zhan, Yan Bi, and Baoan Ji

26. Pancreatic Acinar to Ductal Metaplasia and Pancreatic Cancer

Liang Wang, Dacheng Xie, and Daoyan Wei

27. Orthotopic Pancreatic Tumor Mouse Models of Liver Metastasis

George Zhang and Yi-Chieh Nancy Du

28. Molecular and Physiological Evaluation of Pancreatic Cancer-Induced Cachexia

Index….