Ovarian Cancer, 1st ed. 2022 Methods and Protocols Methods in Molecular Biology Series, Vol. 2424

Part I: Clinical Perspectives

1. Clinical Staging of Ovarian Cancer

            Andrea S. O’Shea

2. Pathologic Classification of Ovarian Cancer

            Stephanie M. McGregor

Part II: Tumor Microenvironment

3. Multiparameter Flow Cytometry for Detailed Characterization of Peritoneal Immune Cells from Patients with Ovarian Cancer

            Jessica Vazquez, Dagna Sheerar, Aleksandar K. Stanic, and Manish S. Patankar

4. Mass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumors

            Veronica D. Gonzalez, Ying-Wen Huang, and Wendy J. Fantl

5. Processing and Analysis of Ascites

            Hannah M. Micek, Molly J. Carroll, Lisa Barroilhet, and Pamela K. Kreeger

6. Multispectral Staining and Analysis of Extracellular Matrix

            Carine M. Renner, Mike R. Visetsouk, Pamela K. Kreeger, and Kristyn S. Masters

7. Quantitative Analysis of the Extracellular Matrix by Immunoblot

            Kaitlin C. Fogg

Part III: Cell Isolation

8. Multiparameter Single Cell Characterization of Ovarian Intratumor Heterogeneity

            Kristin G. Beaumont, Christina Andreou, Ethan Ellis, and Robert Sebra

9. Culturing Primary Human Mesothelial Cells

            Mary Mullen, Hollie Noia, and Katherine Fuh

10. Isolation of Normal and Cancer-Associated Fibroblasts

            Katarzyna Zawieracz and Mark A. Eckert

11. Isolation of Primary Normal and Cancer-Associated Adipocytes from the Omentum

            Abir Mukherjee

Part IV: Model Systems

12. Isolation of Fallopian Tube Epithelium and Ovaries for Assessment of Cilia Beating Frequency (CBF)

            Angela Russo and Joanna E. Burdette

13. Ex Vivo Ovarian Culture to Model the Initial Metastasis in Ovarian Cancer

14. In Vivo and Ex Vivo Analysis of Omental Adhesion in Ovarian Cancer

            Elizabeth I. Harper and Tyvette S. Hilliard

15. The Role of the Tumor Microenvironment in CSC Enrichment and Chemoresistance: 3D Co-Culture Methods

            Michael Bregenzer, Eric Horst, Pooja Mehta, Catherine Snyder, Taylor Repetto, and Geeta Mehta

16. Establishment of In Vivo Ovarian Cancer Mouse Models Using Intraperitoneal Tumor Cell Injection

            Sonam Mittal, Prachi Gupta, Pradeep Chaluvally-Raghavan, and Sunila Pradeep

17. Humanized Patient-Derived Xenograft Models of Ovarian Cancer

            Sarah B. Gitto, Erin George, Sergey Medvedev, Fiona Simpkins, and Daniel J. Powell, Jr.

18. Xenograft Models of Ovarian Cancer for Therapy Evaluation

            Mihaela Popa, Vibeke Fosse, Katrin Kleinmanns, Line Bjørge, and Emmet McCormack

19. Confocal Imaging of Single-Cell Signaling in Orthotopic Models of Ovarian Cancer

            Dylan O. Matvey, Thomas S.C. Ng, and Miles A. Miller

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts