Optical Imaging Techniques in Cell Biology (2^nd Ed.)

Optical Imaging Techniques in Cell Biology, Second Edition covers the field of biological microscopy, from the optics of the microscope to the latest advances in imaging below the traditional resolution limit. It includes the techniques?such as labeling by immunofluorescence and fluorescent proteins?which have revolutionized cell biology. Quantitative techniques such as lifetime imaging, ratiometric measurement, and photoconversion are all covered in detail.

Expanded with a new chapter and 40 new figures, the second edition has been updated to cover the latest developments in optical imaging techniques. Explanations throughout are accurate, detailed, but as far as possible non-mathematical. This edition includes appendices with useful practical protocols, references, and suggestions for further reading. Color figures are integrated throughout.

The Light Microscope. Optical Contrasting Techniques. Fluorescence and Fluorescence Microscopy. Image Capture. The Confocal Microscope. The Digital Image. Aberrations and Their Consequences. Nonlinear Microscopy. High-Speed Confocal Microscopy. Deconvolution and Image Processing. Three-Dimensional Imaging: Stereoscopy and Reconstruction. Green Fluorescent Protein. Fluorescent Staining. Quantitative Fluorescence. Advanced Fluorescence Techniques: FLIM, FRET, and FCS. Evanescent Wave Microscopy. Beyond the Diffraction Limit. Appendix A: Microscope Care and Maintenance. Appendix B: Keeping Cells Alive under the Microscope. Appendix C: Antibody Labeling of Plant and Animal Cells: Tips and Sample Schedules. Appendix D: Image Processing with ImageJ.

Guy Cox is a professor within the Electron Microscopy Unit at the University of Sydney, Australia.